Targeted next-generation sequencing assay to detect 3 Moraxella spp. directly from bovine ocular swabs

Infectious bovine keratoconjunctivitis (IBK) is associated with 2 species of Moraxella: M. bovis and M. bovoculi. A third novel Moraxella spp., designated tentatively as M. oculi, has been identified from the eyes of cattle with and without pinkeye. These 3 Moraxella spp. can be found in various com...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2024-01, Vol.36 (1), p.120-123
Main Authors: Wilkes, Rebecca P., Kattoor, Jobin J., Weng, Hsin-Yi, Anis, Eman
Format: Article
Language:English
Subjects:
Animals
Brief Reports
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - microbiology
High-Throughput Nucleotide Sequencing - veterinary
Keratoconjunctivitis, Infectious - diagnosis
Keratoconjunctivitis, Infectious - microbiology
Moraxella - genetics
Moraxellaceae Infections - diagnosis
Moraxellaceae Infections - microbiology
Moraxellaceae Infections - veterinary
Mycoplasma Infections - veterinary
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Summary:Infectious bovine keratoconjunctivitis (IBK) is associated with 2 species of Moraxella: M. bovis and M. bovoculi. A third novel Moraxella spp., designated tentatively as M. oculi, has been identified from the eyes of cattle with and without pinkeye. These 3 Moraxella spp. can be found in various combinations within the same clinical sample, making speciation of this genus directly from a sample impossible with Sanger sequencing. Assessing Moraxella diversity found in IBK- and non-IBK–affected cattle eyes, independent of culture, may provide additional information about IBK by avoiding the selectivity bias of culturing. We developed a targeted NGS panel to detect and speciate these 3 Moraxella spp. directly from bovine ocular swabs. Our targeted panel amplifies bacterial essential genes and the 16S-23S ribosomal RNA intergenic spacer region (ITS) of the 3 Moraxella spp. and speciates based on these sequences. Our panel was able to differentiate the 3 species directly from DNA extracted from 13 swabs (6 from healthy animals, 7 from animals with IBK), and every swab except one (clinically healthy eye) had the 3 Moraxella spp. Targeted NGS with sequencing of Moraxella spp. housekeeping genes appears to be a suitable method for speciation of Moraxella directly from ocular swabs.
ISSN:1040-6387
1943-4936
1943-4936
DOI:10.1177/10406387231216698