Assessment of copper accumulation in archived liver specimens from cats

Objectives The aim of this study was to assess hepatic copper concentrations and zonal distribution in cat liver specimens. Methods For this study, 121 archived, formalin-fixed, paraffin-embedded liver specimens from cats were used. Tissue sections were stained for copper with rhodanine and scored f...

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Bibliographic Details
Published in:Journal of feline medicine and surgery 2021-06, Vol.23 (6), p.526-533
Main Authors: Yamkate, Punyamanee, Gold, Randi M, Xenoulis, Panagiotis G, Steiger, Katja, Twedt, David C, Suchodolski, Jan S, Steiner, Joerg M, Lidbury, Jonathan A
Format: Article
Language:English
Subjects:
Animals
Cat Diseases
Cats
Copper
Fatty Liver - veterinary
Liver
Original
Rhodanine
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Summary:Objectives The aim of this study was to assess hepatic copper concentrations and zonal distribution in cat liver specimens. Methods For this study, 121 archived, formalin-fixed, paraffin-embedded liver specimens from cats were used. Tissue sections were stained for copper with rhodanine and scored from 0 (no copper accumulation) to 5 (panlobular copper accumulation). The tissue specimens were then deparaffinized and hepatic copper concentrations were measured using flame atomic absorption spectroscopy. Results Tissue samples were categorized into four groups based on histopathologic findings: (1) no significant histopathologic hepatic changes (n = 66); (2) hepatic steatosis (n = 18); (3) inflammatory or infectious disease (n = 24); and (4) neoplasia (n = 13). Of the 121 specimens, 13 (11%) stained positive for copper, with three having a score ⩾3. Thirty-seven specimens (31%) had copper concentrations above the reference interval ([RI]
ISSN:1098-612X
1532-2750
DOI:10.1177/1098612X20961350