Biosynthesis and degradation of a wheat embryo cytokinin-binding protein during embryogenesis and germination

The accumulation and degradation of a wheat (Triticum durum) embryo cytokinin-binding protein (CBF-1) was followed during embryo development and germination by its $\text{N}^{6}$-benzyladenine (BA) binding activity and immunological reactivity (rocket immunoelectrophoresis and Western blotting). Bot...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1985-11, Vol.79 (3), p.706-710
Main Authors: Brinegar, A.C, Stevens, A, Fox, J.E
Format: Article
Language:English
Subjects:
Centrifugation
Chromatography
Cytokinins
DEGRADATION
DESARROLLO EMBRIONARIO
DETERIORO
DEVELOPPEMENT EMBRYONNAIRE
Embryogenesis
EMBRYONIC DEVELOPMENT
Embryos
Gels
GERMINACION
GERMINATION
PROTEIN SYNTHESIS
PROTEINAS
PROTEINE
PROTEINS
RNA
SINTESIS DE PROTEINAS
Storage proteins
SYNTHESE PROTEIQUE
TRITICUM DURUM
Western blotting
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Summary:The accumulation and degradation of a wheat (Triticum durum) embryo cytokinin-binding protein (CBF-1) was followed during embryo development and germination by its $\text{N}^{6}$-benzyladenine (BA) binding activity and immunological reactivity (rocket immunoelectrophoresis and Western blotting). Both BA binding activity and CBF-1 appeared at 2 weeks post-anthesis and rose sharply between 2 to 4 weeks before leveling off to approximately 47 micrograms per embryo (9% of the soluble embryo protein at maturity). In vitro translation of polyadenylated RNA from 20-day-old embryos yielded a polypeptide which was immunoprecipitable with anti-CBF-1 IgG and migrated closely to the 54-kilodalton CBF-1 polypeptide on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Upon germination, both the amount of CBF-1 and BA binding activity dropped to low levels within 3 days. The data are discussed in relation to the possible role of CBF-1 as a regulator of cytokinin availability, and comparisons are drawn between the structural and biosynthetic similarities found between CBF-1 and the vicilin storage proteins of legumes. An improved method for isolating undegraded CBF-1 from whole seeds is also presented.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.79.3.706