Alterations in Nicotiana tabacum L. cv Xanthi cell membrane function following treatment with an ethylene biosynthesis-inducing endoxylanase

An ethylene biosynthesis-inducing xylanase (EIX) produced by the fungus Trichoderma viride elicited enhanced ethylene biosynthesis and leakage of potassium and other cellular components when applied to leaf disks of tobacco (Nicotiana tabacum L. cv Xanthi). Suspension-cultured cells of Xanthi tobacc...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-10, Vol.100 (2), p.749-755
Main Authors: Bailey, B.A, Korcak, R.F, Anderson, J.D
Format: Article
Language:English
Subjects:
Agriculture
Agronomy. Soil science and plant productions
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
Biosynthesis
CALCIO
CALCIUM
CATION
CATIONES
Cell culture techniques
Cell membranes
Cell walls
CULTIVO DE CELULAS
CULTURE DE CELLULE
Cultured cells
effects on
Electrolytes
endoxylanase
ETHYLENE
Ethylene production
ETILENO
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
HIDROLASAS
HYDROLASE
ION
IONES
MEMBRANAS CELULARES
MEMBRANE CELLULAIRE
Microbe-Plant Interactions
NICOTIANA TABACUM
Pathology, epidemiology, host-fungus relationships. Damages, economic importance
PERMEABILIDAD
PERMEABILITE
Phytopathology. Animal pests. Plant and forest protection
Plant cells
Plants
POTASIO
POTASSIUM
SUBSTANCE DE CROISSANCE VEGETALE
SUSTANCIAS DE CRECIMIENTO VEGETAL
transport
TRICHODERMA VIRIDE
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Summary:An ethylene biosynthesis-inducing xylanase (EIX) produced by the fungus Trichoderma viride elicited enhanced ethylene biosynthesis and leakage of potassium and other cellular components when applied to leaf disks of tobacco (Nicotiana tabacum L. cv Xanthi). Suspension-cultured cells of Xanthi tobacco responded to EIX by rapid efflux of potassium, uptake of calcium, alkalization of the medium, inhibition of ethylene biosynthesis, and increased leakage of cellular components. EIX-treated cell suspensions released 1-aminocyclopropane-1-carboxylate (ACC) into the surrounding medium, resulting in a reduction of cellular pools of ACC. The responses of both cell suspensions and leaf disks were inhibited (50-80%) by the preincubation of the tissues with the calcium channel blocker La3+. High concentrations of EGTA inhibited the alkalization of the medium by cell suspensions responding to EIX, but EGTA alone caused extensive loss of K+ and ACC and inhibited ethylene biosynthesis by tobacco cells. Alterations in membrane function appear to be important in the mode of action of EIX in Xanthi cells
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.100.2.749