Release of small polyuronides from Nitella cell walls during ionic exchange

Mono-divalent ion exchange in isolated cell walls of Nitella flexilis (L.) Ag. induces a marked loss of wall polymers and a decrease in the wall cationic exchange capacity. These data correlate with the replacement in the walls of adsorbed Mn2+ by Na+ ions. Boiling wall samples in methanol for 1 h o...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-10, Vol.100 (2), p.846-852
Main Authors: Gillet, C, Cambier, P, Liners, F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell biochemistry
Cell physiology
Cell walls
Cellular and Structural Biology
Charophyceae
ECHANGE D'ION
Enzyme linked immunosorbent assay
Freshwater
Fundamental and applied biological sciences. Psychology
Hydrolysis
INTERCAMBIO IONICO
LESSIVAGE DU SOL
LIXIVIACION
Monoclonal antibodies
NITELLA
Nitella flexilis
PARED CELULAR
PAROI CELLULAIRE
PECTINAS
PECTINE
Plant cells
Plant physiology and development
Plants
POLIURONIDOS
Polymers
Polysaccharides
POLYURONIDE
Sugars
Uronic acids
XILANOS
XYLANE
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Summary:Mono-divalent ion exchange in isolated cell walls of Nitella flexilis (L.) Ag. induces a marked loss of wall polymers and a decrease in the wall cationic exchange capacity. These data correlate with the replacement in the walls of adsorbed Mn2+ by Na+ ions. Boiling wall samples in methanol for 1 h or keeping the ionic solutions chilled to 4 degrees C does not inhibit the cell wall polymer leakage but modifies the kinetics both of the ionic exchange and of the released polymers. These data are more compatible with physical rather than enzymic induced processes. The extracted polymers in the successively renewed NaCl solutions initially belong to the wall protein and pectin fractions and mainly to pectic fractions subsequently. Determination of the average degree of polymerization shows that the average molecular size of the lost acidic polysaccharides increases with extraction time up an average polymerization degree of 25. Enzyme-linked immunosorbent assay inhibition tests show the presence of homopolymer blocks equal to or higher than 10 in the released polymer fragments. Compositional analysis of released polysaccharides suggests that the pectin lost by action of monovalent ions was largely composed of rhamnogalacturonans whose acidic residue fraction is approximately 60% in association with galactose chains. Small quantities of glucuroncuronylated xylans are also found
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.100.2.846