Modulation of the Access of Exogenous NAD(P)H to the Alternative Pathway in Potato Tuber Callus Mitochondria with Triton X-100

Alternative oxidase activity in potato tuber (Solanum tuberosum L. cv Bintje) callus mitochondria with exogenous NAD(P)H as substrate is inhibited by low concentrations of the detergent Triton X-100. Alternative oxidase activity with succinate or malate as substrate is not affected by these low conc...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-11, Vol.100 (3), p.1259-1262
Main Authors: WAGNER, A. M, VAN DEN BERGEN, C. W. M, KRAB, K
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Callus
Dehydrogenases
Detergents
Fundamental and applied biological sciences. Psychology
Low concentrations
Metabolism
Metabolism and Enzymology
Mitochondria
Oxidases
Oxidation
Oxygen
Photosynthesis, respiration. Anabolism, catabolism
Plant physiology and development
Respiration
Tubers
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Summary:Alternative oxidase activity in potato tuber (Solanum tuberosum L. cv Bintje) callus mitochondria with exogenous NAD(P)H as substrate is inhibited by low concentrations of the detergent Triton X-100. Alternative oxidase activity with succinate or malate as substrate is not affected by these low concentrations of Triton X-100. Cytochrome pathway activity was not influenced under these conditions, neither with endogenous nor with exogenous substrate. Washing of Triton X-100-treated mitochondria did partially restore both uninhibited and CN-resistant NADH oxidation, indicating that under these conditions Triton X-100 does not permanently remove major components from the mitochondrial membrane. Apparently, it is possible to manipulate mitochondria in such a way that the access of exogenous NADH to the alternative pathway is blocked while access to the cytochrome pathway is uninhibited. It is suggested that membrane conditions have a regulatory function (possibly via influencing the diffusion path) in the oxidation of exogenous NADH via the alternative pathway.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.100.3.1259