Detection of a cytosolic glutamine synthetase in leaves of Nicotiana tabacum L. by immunocytochemical methods

Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-11, Vol.100 (3), p.1591-1594
Main Authors: CARVALHO, H, PEREIRA, S, SUNKEL, C, SALEMA, R
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Antibodies
Biological and medical sciences
Chloroplasts
citoplasma
Communications
cytoplasm
cytoplasme
feuille
Fundamental and applied biological sciences. Psychology
hojas
immunologie
immunology
inmunologia
Leaves
ligasas
ligase
ligases
Mesophyll cells
Metabolism
Metabolism and Enzymology
nicotiana tabacum
Nitrogen metabolism
Phloem
Phloem companion cells
Plant cells
Plant physiology and development
Plant tissues
Protein isoforms
Vascular tissues
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.100.3.1591