Loading…
Effect of 2,4-dichlorophenoxyacetic acid on endogenous cyanide, beta-cyanoalanine synthase activity, and ethylene evolution in seedlings of soybean and barley
Treatment of etiolated seedlings of barley (Hordeum vulgare) and soybean (Glycine max) with 1 millimolar 2,4-dichlorophenoxyacetic acid (2,4-D) resulted in a 14-fold and greater than 100-fold increase in ethylene production, respectively. Simultaneous monitoring of endogenous cyanide and beta-cyanoa...
Saved in:
Published in: | Plant physiology (Bethesda) 1990-11, Vol.94 (3), p.1143-1148 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c3663-fe202e0d9b4db02d7078d4ada8453fae7b89d2dce72f88becc205b4a3cb43ba73 |
---|---|
cites | |
container_end_page | 1148 |
container_issue | 3 |
container_start_page | 1143 |
container_title | Plant physiology (Bethesda) |
container_volume | 94 |
creator | Tittle, F.L. (Northern Alberta Institute of Technology, Edmonton, Alberta) Goudey, J.S Spencer, M.S |
description | Treatment of etiolated seedlings of barley (Hordeum vulgare) and soybean (Glycine max) with 1 millimolar 2,4-dichlorophenoxyacetic acid (2,4-D) resulted in a 14-fold and greater than 100-fold increase in ethylene production, respectively. Simultaneous monitoring of endogenous cyanide and beta-cyanoalanine synthase (beta-CAS) (EC 4.4.1.9) activity was also performed. Endogenous levels of cyanide did not change in barley. In soybean, endogenous cyanide increased within 3 hours, increased again 6 hours after exposure to 2,4-D, and continued to increase throughout the experimental period. The activity of beta-CAS increased in both barley and soybean 9 hours after herbicide treatment. The increase in cyanide preceded the increase in beta-CAS activity by 3 to 6 hours in soybean. The steady-state concentration of endogenous cyanide in soybean was 1 micromolar, based on rates of ethylene production and cyanide metabolism by beta-CAS. This agreed with the determination of endogenous cyanide by both distillation and isotope dilution. Given the apparent compartmentalization of beta-CAS in mitochondria and the localization of ethylene/HCN production at the plasmalemma and/or tonoplast, our results suggest that extra-mitochondrial accumulation of cyanide in the cytoplasm may occur. If so, the activity of cyanide-sensitive cytoplasmic enzymes could be adversely affected, thus possibly contributing to the toxicity of 2,4-D |
doi_str_mv | 10.1104/pp.94.3.1143 |
format | article |
fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1077354</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4273213</jstor_id><sourcerecordid>4273213</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3663-fe202e0d9b4db02d7078d4ada8453fae7b89d2dce72f88becc205b4a3cb43ba73</originalsourceid><addsrcrecordid>eNpVkU2P0zAQhiMEYsvCjRNCyBfEpSn-apxckNBq-ZBW4gB7tsb2pPUqtYOdVuTP8FtxabULJ3v8Pn5nNG9VvWR0xRiV78dx1cmVKIUUj6oFWwte87VsH1cLSsudtm13UT3L-Y5SygSTT6sL1jSNamm3qH5f9z3aicSe8KWsnbfbIaY4bjHEXzNYnLwlYL0jMRAMLm6KsM_EzhC8wyUxOEF9rCIM5SkgyXOYtpCxfJv8wU_zkkBwBKftPGDR8RCH_eSLnw8kI7rBh00-TpDjbBDCX9xAGnB-Xj3pYcj44nxeVrefrn9cfalvvn3-evXxpraiaUTdI6ccqeuMdIZyp6hqnQQHrVyLHlCZtnPcWVS8b1uD1nK6NhKENVIYUOKy-nDyHfdmhwUMU4JBj8nvIM06gtf_K8Fv9SYeNKNKibUsBu_OBin-3GOe9M5ni0PZCZZ9aSWE7CRtRCGXJ9KmmHPC_r4Lo_qYqB5H3Ukt9DHRgr_5d7IH-BxhAd6eAcgWhj5BsD4_cJ1qhJJN4V6fuLs8xXSvS64EZ8c-r05yD1HDJhWL2-8dY7yhXPwBbDG__g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733494063</pqid></control><display><type>article</type><title>Effect of 2,4-dichlorophenoxyacetic acid on endogenous cyanide, beta-cyanoalanine synthase activity, and ethylene evolution in seedlings of soybean and barley</title><source>Alma/SFX Local Collection</source><source>JSTOR</source><creator>Tittle, F.L. (Northern Alberta Institute of Technology, Edmonton, Alberta) ; Goudey, J.S ; Spencer, M.S</creator><creatorcontrib>Tittle, F.L. (Northern Alberta Institute of Technology, Edmonton, Alberta) ; Goudey, J.S ; Spencer, M.S</creatorcontrib><description>Treatment of etiolated seedlings of barley (Hordeum vulgare) and soybean (Glycine max) with 1 millimolar 2,4-dichlorophenoxyacetic acid (2,4-D) resulted in a 14-fold and greater than 100-fold increase in ethylene production, respectively. Simultaneous monitoring of endogenous cyanide and beta-cyanoalanine synthase (beta-CAS) (EC 4.4.1.9) activity was also performed. Endogenous levels of cyanide did not change in barley. In soybean, endogenous cyanide increased within 3 hours, increased again 6 hours after exposure to 2,4-D, and continued to increase throughout the experimental period. The activity of beta-CAS increased in both barley and soybean 9 hours after herbicide treatment. The increase in cyanide preceded the increase in beta-CAS activity by 3 to 6 hours in soybean. The steady-state concentration of endogenous cyanide in soybean was 1 micromolar, based on rates of ethylene production and cyanide metabolism by beta-CAS. This agreed with the determination of endogenous cyanide by both distillation and isotope dilution. Given the apparent compartmentalization of beta-CAS in mitochondria and the localization of ethylene/HCN production at the plasmalemma and/or tonoplast, our results suggest that extra-mitochondrial accumulation of cyanide in the cytoplasm may occur. If so, the activity of cyanide-sensitive cytoplasmic enzymes could be adversely affected, thus possibly contributing to the toxicity of 2,4-D</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.94.3.1143</identifier><identifier>PMID: 16667809</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>2,4-D ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ALANINA ; ALANINE ; Barley ; Biological and medical sciences ; CIANUROS ; Cyanides ; CYANURE ; Enzymes ; ETHYLENE ; Ethylene production ; ETILENO ; Fundamental and applied biological sciences. Psychology ; GLYCINE MAX ; Growth regulators ; Herbicides ; HORDEUM VULGARE ; LIGASAS ; LIGASE ; Metabolism ; Metabolism and Enzymology ; METABOLISME ; METABOLISMO ; Nitrates ; Plant growth ; Plant physiology and development ; Plants ; PLANTULAS ; PLANTULE ; PRODUCCION VEGETAL ; PRODUCTION VEGETALE ; Seedlings ; Soybeans</subject><ispartof>Plant physiology (Bethesda), 1990-11, Vol.94 (3), p.1143-1148</ispartof><rights>Copyright 1990 American Society of Plant Physiologists</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3663-fe202e0d9b4db02d7078d4ada8453fae7b89d2dce72f88becc205b4a3cb43ba73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4273213$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4273213$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,885,27915,27916,58229,58462</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19763746$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16667809$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tittle, F.L. (Northern Alberta Institute of Technology, Edmonton, Alberta)</creatorcontrib><creatorcontrib>Goudey, J.S</creatorcontrib><creatorcontrib>Spencer, M.S</creatorcontrib><title>Effect of 2,4-dichlorophenoxyacetic acid on endogenous cyanide, beta-cyanoalanine synthase activity, and ethylene evolution in seedlings of soybean and barley</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Treatment of etiolated seedlings of barley (Hordeum vulgare) and soybean (Glycine max) with 1 millimolar 2,4-dichlorophenoxyacetic acid (2,4-D) resulted in a 14-fold and greater than 100-fold increase in ethylene production, respectively. Simultaneous monitoring of endogenous cyanide and beta-cyanoalanine synthase (beta-CAS) (EC 4.4.1.9) activity was also performed. Endogenous levels of cyanide did not change in barley. In soybean, endogenous cyanide increased within 3 hours, increased again 6 hours after exposure to 2,4-D, and continued to increase throughout the experimental period. The activity of beta-CAS increased in both barley and soybean 9 hours after herbicide treatment. The increase in cyanide preceded the increase in beta-CAS activity by 3 to 6 hours in soybean. The steady-state concentration of endogenous cyanide in soybean was 1 micromolar, based on rates of ethylene production and cyanide metabolism by beta-CAS. This agreed with the determination of endogenous cyanide by both distillation and isotope dilution. Given the apparent compartmentalization of beta-CAS in mitochondria and the localization of ethylene/HCN production at the plasmalemma and/or tonoplast, our results suggest that extra-mitochondrial accumulation of cyanide in the cytoplasm may occur. If so, the activity of cyanide-sensitive cytoplasmic enzymes could be adversely affected, thus possibly contributing to the toxicity of 2,4-D</description><subject>2,4-D</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ALANINA</subject><subject>ALANINE</subject><subject>Barley</subject><subject>Biological and medical sciences</subject><subject>CIANUROS</subject><subject>Cyanides</subject><subject>CYANURE</subject><subject>Enzymes</subject><subject>ETHYLENE</subject><subject>Ethylene production</subject><subject>ETILENO</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLYCINE MAX</subject><subject>Growth regulators</subject><subject>Herbicides</subject><subject>HORDEUM VULGARE</subject><subject>LIGASAS</subject><subject>LIGASE</subject><subject>Metabolism</subject><subject>Metabolism and Enzymology</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>Nitrates</subject><subject>Plant growth</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>PLANTULAS</subject><subject>PLANTULE</subject><subject>PRODUCCION VEGETAL</subject><subject>PRODUCTION VEGETALE</subject><subject>Seedlings</subject><subject>Soybeans</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNpVkU2P0zAQhiMEYsvCjRNCyBfEpSn-apxckNBq-ZBW4gB7tsb2pPUqtYOdVuTP8FtxabULJ3v8Pn5nNG9VvWR0xRiV78dx1cmVKIUUj6oFWwte87VsH1cLSsudtm13UT3L-Y5SygSTT6sL1jSNamm3qH5f9z3aicSe8KWsnbfbIaY4bjHEXzNYnLwlYL0jMRAMLm6KsM_EzhC8wyUxOEF9rCIM5SkgyXOYtpCxfJv8wU_zkkBwBKftPGDR8RCH_eSLnw8kI7rBh00-TpDjbBDCX9xAGnB-Xj3pYcj44nxeVrefrn9cfalvvn3-evXxpraiaUTdI6ccqeuMdIZyp6hqnQQHrVyLHlCZtnPcWVS8b1uD1nK6NhKENVIYUOKy-nDyHfdmhwUMU4JBj8nvIM06gtf_K8Fv9SYeNKNKibUsBu_OBin-3GOe9M5ni0PZCZZ9aSWE7CRtRCGXJ9KmmHPC_r4Lo_qYqB5H3Ukt9DHRgr_5d7IH-BxhAd6eAcgWhj5BsD4_cJ1qhJJN4V6fuLs8xXSvS64EZ8c-r05yD1HDJhWL2-8dY7yhXPwBbDG__g</recordid><startdate>19901101</startdate><enddate>19901101</enddate><creator>Tittle, F.L. (Northern Alberta Institute of Technology, Edmonton, Alberta)</creator><creator>Goudey, J.S</creator><creator>Spencer, M.S</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19901101</creationdate><title>Effect of 2,4-dichlorophenoxyacetic acid on endogenous cyanide, beta-cyanoalanine synthase activity, and ethylene evolution in seedlings of soybean and barley</title><author>Tittle, F.L. (Northern Alberta Institute of Technology, Edmonton, Alberta) ; Goudey, J.S ; Spencer, M.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3663-fe202e0d9b4db02d7078d4ada8453fae7b89d2dce72f88becc205b4a3cb43ba73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>2,4-D</topic><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ALANINA</topic><topic>ALANINE</topic><topic>Barley</topic><topic>Biological and medical sciences</topic><topic>CIANUROS</topic><topic>Cyanides</topic><topic>CYANURE</topic><topic>Enzymes</topic><topic>ETHYLENE</topic><topic>Ethylene production</topic><topic>ETILENO</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLYCINE MAX</topic><topic>Growth regulators</topic><topic>Herbicides</topic><topic>HORDEUM VULGARE</topic><topic>LIGASAS</topic><topic>LIGASE</topic><topic>Metabolism</topic><topic>Metabolism and Enzymology</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>Nitrates</topic><topic>Plant growth</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>PLANTULAS</topic><topic>PLANTULE</topic><topic>PRODUCCION VEGETAL</topic><topic>PRODUCTION VEGETALE</topic><topic>Seedlings</topic><topic>Soybeans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tittle, F.L. (Northern Alberta Institute of Technology, Edmonton, Alberta)</creatorcontrib><creatorcontrib>Goudey, J.S</creatorcontrib><creatorcontrib>Spencer, M.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tittle, F.L. (Northern Alberta Institute of Technology, Edmonton, Alberta)</au><au>Goudey, J.S</au><au>Spencer, M.S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of 2,4-dichlorophenoxyacetic acid on endogenous cyanide, beta-cyanoalanine synthase activity, and ethylene evolution in seedlings of soybean and barley</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1990-11-01</date><risdate>1990</risdate><volume>94</volume><issue>3</issue><spage>1143</spage><epage>1148</epage><pages>1143-1148</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Treatment of etiolated seedlings of barley (Hordeum vulgare) and soybean (Glycine max) with 1 millimolar 2,4-dichlorophenoxyacetic acid (2,4-D) resulted in a 14-fold and greater than 100-fold increase in ethylene production, respectively. Simultaneous monitoring of endogenous cyanide and beta-cyanoalanine synthase (beta-CAS) (EC 4.4.1.9) activity was also performed. Endogenous levels of cyanide did not change in barley. In soybean, endogenous cyanide increased within 3 hours, increased again 6 hours after exposure to 2,4-D, and continued to increase throughout the experimental period. The activity of beta-CAS increased in both barley and soybean 9 hours after herbicide treatment. The increase in cyanide preceded the increase in beta-CAS activity by 3 to 6 hours in soybean. The steady-state concentration of endogenous cyanide in soybean was 1 micromolar, based on rates of ethylene production and cyanide metabolism by beta-CAS. This agreed with the determination of endogenous cyanide by both distillation and isotope dilution. Given the apparent compartmentalization of beta-CAS in mitochondria and the localization of ethylene/HCN production at the plasmalemma and/or tonoplast, our results suggest that extra-mitochondrial accumulation of cyanide in the cytoplasm may occur. If so, the activity of cyanide-sensitive cytoplasmic enzymes could be adversely affected, thus possibly contributing to the toxicity of 2,4-D</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16667809</pmid><doi>10.1104/pp.94.3.1143</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0032-0889 |
ispartof | Plant physiology (Bethesda), 1990-11, Vol.94 (3), p.1143-1148 |
issn | 0032-0889 1532-2548 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1077354 |
source | Alma/SFX Local Collection; JSTOR |
subjects | 2,4-D ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ALANINA ALANINE Barley Biological and medical sciences CIANUROS Cyanides CYANURE Enzymes ETHYLENE Ethylene production ETILENO Fundamental and applied biological sciences. Psychology GLYCINE MAX Growth regulators Herbicides HORDEUM VULGARE LIGASAS LIGASE Metabolism Metabolism and Enzymology METABOLISME METABOLISMO Nitrates Plant growth Plant physiology and development Plants PLANTULAS PLANTULE PRODUCCION VEGETAL PRODUCTION VEGETALE Seedlings Soybeans |
title | Effect of 2,4-dichlorophenoxyacetic acid on endogenous cyanide, beta-cyanoalanine synthase activity, and ethylene evolution in seedlings of soybean and barley |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T23%3A53%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%202,4-dichlorophenoxyacetic%20acid%20on%20endogenous%20cyanide,%20beta-cyanoalanine%20synthase%20activity,%20and%20ethylene%20evolution%20in%20seedlings%20of%20soybean%20and%20barley&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Tittle,%20F.L.%20(Northern%20Alberta%20Institute%20of%20Technology,%20Edmonton,%20Alberta)&rft.date=1990-11-01&rft.volume=94&rft.issue=3&rft.spage=1143&rft.epage=1148&rft.pages=1143-1148&rft.issn=0032-0889&rft.eissn=1532-2548&rft.coden=PPHYA5&rft_id=info:doi/10.1104/pp.94.3.1143&rft_dat=%3Cjstor_pubme%3E4273213%3C/jstor_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3663-fe202e0d9b4db02d7078d4ada8453fae7b89d2dce72f88becc205b4a3cb43ba73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=733494063&rft_id=info:pmid/16667809&rft_jstor_id=4273213&rfr_iscdi=true |