Isolation of a cDNA clone for alpha-amylase in mung bean cotyledons. Analysis of alpha-amylase mRNA levels in cotyledons during and following germination of mung bean seeds

A cDNA was isolated that codes for alpha-amylase in mung bean (Vigna radiata) cotyledons, and the nucleotide sequence was determined. The deduced amino acid sequence (421 amino acid residues) is about 65% homologous with those of barley alpha-amylases. By comparing the deduced sequence with the sequ...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1990-11, Vol.94 (3), p.1488-1491
Main Authors: Koizuka, N. (Tokyo University of Agriculture and Technology, Tokyo, Japan), Tanaka, Y, Morohashi, Y
Format: Article
Language:English
Subjects:
ALFA AMILASA
ALPHA AMYLASE
Amino acids
Barley
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
CLONE
CLONES
CODE GENETIQUE
CODIGO GENETICO
Communications
Complementary DNA
COTILEDONES
COTYLEDON
Cotyledons
DNA
Enzymes
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENETICA
GENETIQUE
Germination
Imbibition
Messenger RNA
Metabolism
Molecular Biology and Gene Regulation
Plant physiology and development
RNA
Testa
VIGNA RADIATA
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Summary:A cDNA was isolated that codes for alpha-amylase in mung bean (Vigna radiata) cotyledons, and the nucleotide sequence was determined. The deduced amino acid sequence (421 amino acid residues) is about 65% homologous with those of barley alpha-amylases. By comparing the deduced sequence with the sequence of the purified alpha-amylase, it was inferred that 23 N-terminal amino acids of a nascent polypeptide represent a signal peptide. Northern blot analysis showed that the levels of alpha-amylase mRNA are in parallel with the activities of alpha-amylase synthesis in cotyledons. Under the conditions where the solute leakage from cotyledons is accelerated during inhibition, a rapid increase in the amount of the alpha-amylase mRNA occurs. We postulate that a factor(s) which regulates in an inhibitory manner the alpha-amylase expression at the transcriptional level may be present in dry cotyledons and be removed by leakage
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.94.3.1488