Isoelectric focusing of cassava protoplasts

Cassava (Manihot esculenta Crantz) protoplast was analyzed by using isoelectric focusing techniques. Two populations, representing 68 and 32% of the total sample, with mean isoelectric points of 4.48 and 4.60, were obtained using mesophyll protoplasts. The use of this technique allows demonstration...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1991-01, Vol.95 (1), p.335-336
Main Authors: Santana, M.A. (Instituto Internacional de Estudios Avanzados, Caracas, Venezuela), Villegas, L
Format: Article
Language:English
Subjects:
Acetates
Biological and medical sciences
Cassava
Cell fusion
Cell walls
Cellular and Structural Biology
Communications
Dextrans
Fundamental and applied biological sciences. Psychology
ISOELECTROFOCALISATION
ISOELECTROFOQUE
MANIHOT ESCULENTA
Mesophyll
Plant cells
Plant physiology and development
PROPIEDADES FISICO-QUIMICAS
PROPRIETE PHYSICOCHIMIQUE
PROTOPLASTE
PROTOPLASTOS
Protoplasts
Tissue cultures, protoplasts
Viability
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Description
Summary:Cassava (Manihot esculenta Crantz) protoplast was analyzed by using isoelectric focusing techniques. Two populations, representing 68 and 32% of the total sample, with mean isoelectric points of 4.48 and 4.60, were obtained using mesophyll protoplasts. The use of this technique allows demonstration of a discontinuous distribution of protoplast isoelectric point from one species according to their surface potential
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.95.1.335