Sequence analysis and comparison of avocado fruit and bean abscission cellulases

A 1700 nucleotide cDNA clone for a bean (Phaseolus vulgaris cv Red Kidney) abscission cellulase (endo-(1,4)-beta-D-glucanase) has been identified and sequenced. This cDNA clone contains a 1485 nucleotide open reading frame which includes coding sequences for a putative signal peptide and mature prot...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1991-03, Vol.95 (3), p.928-933
Main Authors: Tucker, M.L. (USDA, ARS, Plant Molecular Biology Laboratory, Beltsville, MD), Milligan, S.B
Format: Article
Language:English
Subjects:
ABSCISION
ABSCISSION
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Amino acids
CELLULASE
CELULASA
CODE GENETIQUE
CODIGO GENETICO
Complementary DNA
DNA
Exact sciences and technology
FRUIT
FRUTO
genes
Genomics
GRAINE
Mathematical analysis
Mathematics
Molecular Biology and Gene Regulation
Nucleic acid hybridization
NUCLEOTIDE
Nucleotide sequences
Nucleotides
NUCLEOTIDOS
PERSEA AMERICANA
PHASEOLUS
Phaseolus vulgaris
Polymerase chain reaction
Potential theory
Sciences and techniques of general use
SEMILLA
Sequence analysis
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
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Summary:A 1700 nucleotide cDNA clone for a bean (Phaseolus vulgaris cv Red Kidney) abscission cellulase (endo-(1,4)-beta-D-glucanase) has been identified and sequenced. This cDNA clone contains a 1485 nucleotide open reading frame which includes coding sequences for a putative signal peptide and mature protein. The nucleotide and deduced amino acid sequences for the bean abscission cellulase are compared to the previously reported sequences of an avocado fruit ripening cellulase. Optimal alignment of these sequences shows 64% and 50% identically matched nucleotides and amino acids, respectively. Analysis of the deduced amino acid sequences for the mature bean and avocado cellulases indicates that these two proteins share similar molecular weights, position of cysteine residues, and hydropathic character, but have very different isoelectric points and glycosylation. Genomic blot data suggest that the avocado fruit cellulase belongs to a small gene family, whereas the bean abscission cellulase appears to be encoded by a single gene or a few very closely related genes
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.95.3.928