Transformation of Nicotiana paniculata L., a recalcitrant species, using a T-DNA construct carrying two WUSCHEL-related homeobox genes

A binary vector carrying two WUSCHEL-related homeobox (WOX) genes, WOX2 and WOX8, under the control of a chemical-inducible expression system, worked in the transformation in N. paniculata, a recalcitrant species of Nicotiana. The resulting transformants exhibited improved culture performance in reg...

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Bibliographic Details
Published in:Plant Biotechnology 2023/03/25, Vol.40(1), pp.83-86
Main Authors: Kyo, Masaharu, Hagiya, Momoko, Tada, Madoka, Matsura, Akemi
Format: Article
Language:English
Subjects:
Nicotiana paniculata
Short Communication
WOX2
WOX8
WUSCHEL-related homeobox
XVE system
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Summary:A binary vector carrying two WUSCHEL-related homeobox (WOX) genes, WOX2 and WOX8, under the control of a chemical-inducible expression system, worked in the transformation in N. paniculata, a recalcitrant species of Nicotiana. The resulting transformants exhibited improved culture performance in regeneration from leaf segments and suspended cells. Multicellular masses generated from freely suspended cells showed a specific cell division pattern similar to that of somatic embryo, likely owing to the function of the two WOX genes.
ISSN:1342-4580
1347-6114
DOI:10.5511/plantbiotechnology.22.1124a