Study of the Effect of Wild-Type and Transiently Expressing CXCR4 and IL-10 Mesenchymal Stromal Cells in a Mouse Model of Peritonitis

Sepsis due to peritonitis is a process associated with an inflammatory state. Mesenchymal stromal cells (MSCs) modulate the immune system due to the paracrine factors released and may be a therapeutic alternative. Three treatment groups were developed in a murine model of peritonitis to verify the e...

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Published in:International journal of molecular sciences 2023-12, Vol.25 (1), p.520
Main Authors: Garcia Gómez-Heras, Soledad, Garcia-Arranz, Mariano, Vega-Clemente, Luz, Olivera-Salazar, Rocio, Vélez Pinto, Juan Felipe, Fernández-García, María, Guadalajara, Héctor, Yáñez, Rosa, Garcia-Olmo, Damian
Format: Article
Language:English
Subjects:
Abdomen
Animals
Anti-Inflammatory Agents
Antibiotics
Antimicrobial agents
Chemokines
Cytokines
Disease Models, Animal
Genetically modified organisms
Health aspects
Humans
Immune system
Infection
Inflammation
Interleukin-10 - genetics
Mesenchymal Stem Cells
Mice
Microorganisms
Mortality
Neutrophils
Peritonitis
Receptors, CXCR4
Sepsis
Sepsis - therapy
Stem cells
Surgery
Tumor necrosis factor-TNF
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Summary:Sepsis due to peritonitis is a process associated with an inflammatory state. Mesenchymal stromal cells (MSCs) modulate the immune system due to the paracrine factors released and may be a therapeutic alternative. Three treatment groups were developed in a murine model of peritonitis to verify the effect of human adipose mesenchymal stem cell (hASCs). Additionally, a temporary modification was carried out on them to improve their arrival in inflamed tissues (CXCR4), as well as their anti-inflammatory activity (IL-10). The capacity to reduce systemic inflammation was studied using a local application (peritoneal injection) as a treatment route. Comparisons involving the therapeutic effect of wild-type ASCs and ASCs transiently expressing CXCR4 and IL-10 were carried out with the aim of generating an improved anti-inflammatory response for sepsis in addition to standard antibiotic treatment. However, under the experimental conditions used in these studies, no differences were found between both groups with ASCs. The peritoneal administration of hASCs or genetically modified hASCs constitutes an efficient and safe therapy in our model of mouse peritonitis.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25010520