Elevated Expression of HSP72 in the Prefrontal Cortex and Hippocampus of Rats Subjected to Chronic Mild Stress and Treated with Imipramine

The HSP70 and HSP90 family members belong to molecular chaperones that exhibit protective functions during the cellular response to stressful agents. We investigated whether the exposure of rats to chronic mild stress (CMS), a validated model of depression, affects the expression of HSP70 and HSP90...

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Published in:International journal of molecular sciences 2023-12, Vol.25 (1), p.243
Main Authors: Bielawski, Adam, Zelek-Molik, Agnieszka, Rafa-Zabłocka, Katarzyna, Kowalska, Marta, Gruca, Piotr, Papp, Mariusz, Nalepa, Irena
Format: Article
Language:English
Subjects:
Animals
Antidepressive Agents - pharmacology
Brain
Heat shock proteins
Hippocampus
HSP70 Heat-Shock Proteins
HSP90 Heat-Shock Proteins - genetics
Imipramine
Imipramine - pharmacology
Male
Mediation
Mental depression
Molecular Chaperones
Neurons
Prefrontal Cortex
Protein expression
Rats
Rats, Wistar
RNA
RNA, Messenger - genetics
Sucrose
Type 2 diabetes
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Summary:The HSP70 and HSP90 family members belong to molecular chaperones that exhibit protective functions during the cellular response to stressful agents. We investigated whether the exposure of rats to chronic mild stress (CMS), a validated model of depression, affects the expression of HSP70 and HSP90 in the prefrontal cortex (PFC), hippocampus (HIP) and thalamus (Thal). Male Wistar rats were exposed to CMS for 3 or 8 weeks. The antidepressant imipramine (IMI, 10 mg/kg, i.p., daily) was introduced in the last five weeks of the long-term CMS procedure. Depressive-like behavior was verified by the sucrose consumption test. The expression of mRNA and protein was quantified by real-time PCR and Western blot, respectively. In the 8-week CMS model, stress alone elevated HSP72 and HSP90B mRNA expression in the HIP. HSP72 mRNA was increased in the PFC and HIP of rats not responding to IMI treatment vs. IMI responders. The CMS exposure increased HSP72 protein expression in the cytosolic fraction of the PFC and HIP, and this effect was diminished by IMI treatment. Our results suggest that elevated levels of HSP72 may serve as an important indicator of neuronal stress reactions accompanying depression pathology and could be a potential target for antidepressant strategy.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25010243