Imaging of brain clearance pathways via MRI assessment of the glymphatic system

Glymphatic clearance dysfunction may play an important role in a variety of neurodegenerative diseases and the progression of ageing. However, imaging of the glymphatic system is challenging. In this study, we describe an MRI method based on chemical exchange saturation transfer (CEST) of the Angiop...

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Bibliographic Details
Published in:Aging (Albany, NY.) NY.), 2023-12, Vol.15 (24), p.14945-14956
Main Authors: He, Yi, Guan, Jitian, Lai, Lingfeng, Zhang, Xiaolei, Chen, Beibei, Wang, Xueqing, Wu, Renhua
Format: Article
Language:English
Subjects:
Animals
Brain - diagnostic imaging
Brain - metabolism
Glymphatic System - diagnostic imaging
Lymph Nodes
Lymphatic Vessels - metabolism
Magnetic Resonance Imaging - methods
Rats
Research Paper
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Summary:Glymphatic clearance dysfunction may play an important role in a variety of neurodegenerative diseases and the progression of ageing. However, imaging of the glymphatic system is challenging. In this study, we describe an MRI method based on chemical exchange saturation transfer (CEST) of the Angiopep-2 probe to visualize the clearance function of the glymphatic system. We injected rats with Angiopep-2 via the tail vein and performed MRI at 7 T to track differences in Angiopep-2 signal changes; we then applied the same principles in a bilateral deep cervical lymph node ligation rat model and in ageing rats. We demonstrated the feasibility of Angiopep-2 CEST for visualizing the clearance function of the glymphatic system. Finally, a pathological assessment was performed. Within the model group, the deep cervical lymph node ligation group and the ageing group showed higher CEST signal than the control group. We conclude that this new MRI method can visualize clearance in the glymphatic system.
ISSN:1945-4589
1945-4589
DOI:10.18632/aging.205322