Accurate magnification determination for cryoEM using gold

Determining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximize resolution and enable accurate model building. Here we describe a simple and rapid procedure for determining the absolute magnification in an electron cryomicroscope to a precision of

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Bibliographic Details
Published in:Ultramicroscopy 2024-02, Vol.256, p.113883-None, Article 113883
Main Authors: Dickerson, Joshua L., Leahy, Erin, Peet, Mathew J., Naydenova, Katerina, Russo, Christopher J.
Format: Article
Language:English
Subjects:
CryoEM
Electron cryomicroscopy
Gold
Magnification calibration
Pixel size
Protein structure
Single-particle reconstruction
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Description
Summary:Determining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximize resolution and enable accurate model building. Here we describe a simple and rapid procedure for determining the absolute magnification in an electron cryomicroscope to a precision of
ISSN:0304-3991
1879-2723
1879-2723
DOI:10.1016/j.ultramic.2023.113883