Comprehensive biochemical approach for understanding the interaction between host “common bean” and pathogen “Colletotrichum lindemuthianum” causing bean anthracnose

• Published in: Physiology and molecular biology of plants 2023-12, Vol.29 (12), p.2005-2020
• Main Authors: Shafi, Safoora, Khan, Mohd Anwar, Wani, Fehim Jeelani, Sheikh, Farooq Ahmad, Ganai, Shabir Ahmad, Mughal, Najeeb M, Shikari, Asif Bashir, Varshney, Rajeev Kumar, Djalovic, Ivica, Mir, Reyazul Rouf
• Format: Article
• Language: English
• Subjects: Anthracnose; Ascorbic acid; Beans; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Catalase; Cell Biology; Cell membranes; Colletotrichum lindemuthianum; Comparative studies; Enzymatic activity; Enzyme activity; Enzymes; Flavonoids; Fungal diseases; Fungi; Genotype & phenotype; Genotypes; Hydrogen peroxide; Inoculation; L-Ascorbate peroxidase; Leaves; Life Sciences; Lipid peroxidation; Lipids; Oxygen; Pathogens; Peroxidase; Peroxidation; Phaseolus vulgaris; Phenols; Plant Physiology; Plant Sciences; Plant tissues; Plants (botany); Reactive oxygen species; Research Article; Seeds; Stems
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-023-01394-6

Anthracnose (ANT) caused by Colletotrichum lindemuthianum is the most devastating seed-borne fungal disease of common bean. In response to fungal infections, it is hypothesized that pathogen–plant interactions typically cause hypersensitive reactions by producing reactive oxygen species, hydrogen peroxide and lipid peroxidation of cell membranes. esent study was conducted by inoculating susceptible bean genotype “SB174” and resistant bean genotype “E10” with pathogen “ C. lindemuthianum ”. Defense-related enzymes (ascorbate peroxidase, peroxidase, lipid peroxidase, and catalase) and C-based compounds (total phenols and flavonoids) were studied using the detached bean leaf method. Comparative defense response was studied in different plant tissues (pod, stem, and seed) in susceptible and resistant bean genotypes under uninoculated and pathogen-inoculated conditions. The host‒pathogen interaction was studied at mock inoculation, 2, 4 and 6 days after inoculation (dai). Comparing the pathogen-inoculated bean leaves to water-treated bean leaves, defense enzymes as well as total phenols and flavonoids exhibited differential expression. In a comparative study, the enzyme activity also displayed differential biochemical responses in pods, stems and seeds in both contrasting genotypes. For example, 5.1-fold (pod), 1.5-fold (stem) and 1.06-fold (seed) increases in ascorbate peroxidase activity were observed in the susceptible genotype at 6 dai compared to mock inoculation. Similarly, catalase activity in pods was upregulated (1.47-fold) in the resistant genotype and downregulated (1.30-fold) in the susceptible genotype at 6 dai. The study revealed that defense-related antioxidative enzymes, phenols and flavonoids are fine-tuned to detoxify important reactive oxygen species (ROS) molecules, induce systemic resistance and are successfully controlled in common bean plants against pathogen invasion.