A pilot study to characterize substrate utilization during the fasted state using plasma high-resolution metabolomics

•Indirect calorimetry provides limited information on macronutrient metabolism•Metabolomics provides a global measure of whole-body metabolism•A metabolome-wide association study was performed to characterize fasting metabolism•The fasted plasma metabolome includes diverse pathways and metabolites•M...

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Bibliographic Details
Published in:Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2023-07, Vol.116, p.112160-112160, Article 112160
Main Authors: Taibl, Kaitlin R., Bellissimo, Moriah P., Smith, Matthew Ryan, Liu, Ken H., Tran, ViLinh T., Jones, Dean P., Ziegler, Thomas R., Alvarez, Jessica A.
Format: Article
Language:English
Subjects:
energy
indirect calorimetry
metabolism
metabolomics
nutrition
substrate utilization
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Summary:•Indirect calorimetry provides limited information on macronutrient metabolism•Metabolomics provides a global measure of whole-body metabolism•A metabolome-wide association study was performed to characterize fasting metabolism•The fasted plasma metabolome includes diverse pathways and metabolites•Metabolomics may be a useful tool to profile fasting metabolism in clinical settings High-resolution metabolomics enables global assessment of metabolites and molecular pathways underlying physiological processes, including substrate utilization during the fasted state. The clinical index for substrate utilization, respiratory exchange ratio (RER), is measured via indirect calorimetry. This pilot study used metabolomics to identify metabolic pathways and plasma metabolites associated with substrate utilization in healthy, fasted adults. This cross-sectional study included 33 adults (mean age 27.7 ± 4.9 y, mean BMI=24.8 ± 4.0 kg/m2). Participants underwent indirect calorimetry to determine resting RER following an overnight fast. Untargeted metabolomics was performed on fasted plasma samples using dual column liquid chromatography and ultra-high-resolution mass spectrometry. Linear regression and pathway enrichment analyses defined pathways and metabolites associated with substrate utilization measured with indirect calorimetry. RER was significantly associated with 1,389 metabolites, which were enriched within 13 metabolic pathways (p
ISSN:0899-9007
1873-1244
DOI:10.1016/j.nut.2023.112160