Antimicrobial resistance in multistate outbreaks of nontyphoidal Salmonella infections linked to animal contact-United States, 2015-2018

• Published in: Journal of clinical microbiology 2024-01, Vol.62 (1), p.e0098123
• Main Authors: Frey, Erin, Stapleton, G Sean, Nichols, Megin C, Gollarza, Lauren M, Birhane, Meseret, Chen, Jessica C, McCullough, Andre, Carleton, Heather A, Trees, Eija, Hise, Kelley B, Tolar, Beth, Francois Watkins, Louise
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Cattle; Disease Outbreaks; Drug Resistance, Bacterial; Epidemiology; Guinea Pigs; Microbial Sensitivity Tests; Poultry; Salmonella Infections - epidemiology; Salmonella Infections, Animal - epidemiology; United States - epidemiology
• ISSN: 0095-1137; 1098-660X; 1098-660X
• DOI: 10.1128/jcm.00981-23

Animal contact is an established risk factor for nontyphoidal infections and outbreaks. During 2015-2018, the U.S. Centers for Disease Control and Prevention (CDC) and other U.S. public health laboratories began implementing whole-genome sequencing (WGS) of isolates. WGS was used to supplement the traditional methods of pulsed-field gel electrophoresis for isolate subtyping, outbreak detection, and antimicrobial susceptibility testing (AST) for the detection of resistance. We characterized the epidemiology and antimicrobial resistance (AMR) of multistate salmonellosis outbreaks linked to animal contact during this time period. An isolate was considered resistant if AST yielded a resistant (or intermediate, for ciprofloxacin) interpretation to any antimicrobial tested by the CDC or if WGS showed a resistance determinant in its genome for one of these agents. We identified 31 outbreaks linked to contact with poultry ( = 23), reptiles ( = 6), dairy calves ( = 1), and guinea pigs ( = 1). Of the 26 outbreaks with resistance data available, we identified antimicrobial resistance in at least one isolate from 20 outbreaks (77%). Of 1,309 isolates with resistance information, 247 (19%) were resistant to ≥1 antimicrobial, and 134 (10%) were multidrug-resistant to antimicrobials from ≥3 antimicrobial classes. The use of resistance data predicted from WGS increased the number of isolates with resistance information available fivefold compared with AST, and 28 of 43 total resistance patterns were identified exclusively by WGS; concordance was high (>99%) for resistance determined by AST and WGS. The use of predicted resistance from WGS enhanced the characterization of the resistance profiles of outbreaks linked to animal contact by providing resistance information for more isolates.