Loading…
Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production
2,3-Butanediol (2,3-BDO) is an important gateway molecule for many chemical derivatives. Currently, microbial production is gradually being recognized as a green and sustainable alternative to petrochemical synthesis, but the titer, yield, and productivity of microbial 2,3-BDO remain suboptimal. Her...
Saved in:
| Published in: | Applied microbiology and biotechnology 2024-12, Vol.108 (1), p.146-146, Article 146 |
|---|---|
| Main Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | cdi_FETCH-LOGICAL-c426t-819e32947e5771e50efdfadb8036f56d0b380dada5d0118e82329bf55d90905c3 |
| container_end_page | 146 |
| container_issue | 1 |
| container_start_page | 146 |
| container_title | Applied microbiology and biotechnology |
| container_volume | 108 |
| creator | Lu, Ping Bai, Ruoxuan Gao, Ting Chen, Jiale Jiang, Ke Zhu, Yalun Lu, Ye Zhang, Shuting Xu, Fangxu Zhao, Hongxin |
| description | 2,3-Butanediol (2,3-BDO) is an important gateway molecule for many chemical derivatives. Currently, microbial production is gradually being recognized as a green and sustainable alternative to petrochemical synthesis, but the titer, yield, and productivity of microbial 2,3-BDO remain suboptimal. Here, we used systemic metabolic engineering strategies to debottleneck the 2,3-BDO production in
Enterobacter aerogenes
. Firstly, the pyruvate metabolic network was reconstructed by deleting genes for by-product synthesis to improve the flux toward 2,3-BDO synthesis, which resulted in a 90% increase of the product titer. Secondly, the 2,3-BDO productivity of the IAM1183-LPCT/D was increased by 55% due to the heterologous expression of DR1558 which boosted cell resistance to abiotic stress. Thirdly, carbon sources were optimized to further improve the yield of target products. The IAM1183-LPCT/D showed the highest titer of 2,3-BDO from sucrose, 20% higher than that from glucose, and the yield of 2,3-BDO reached 0.49 g/g. Finally, the titer of 2,3-BDO of IAM1183-LPCT/D in a 5-L fermenter reached 22.93 g/L, 85% higher than the wild-type strain, and the titer of by-products except ethanol was very low.
Key points
Deletion of five key genes in E. aerogenes improved 2,3-BDO production
The titer of 2,3-BDO was increased by 90% by regulating metabolic flux
Response regulator DR1558 was expressed to increase 2,3-BDO productivity
Graphical abstract |
| doi_str_mv | 10.1007/s00253-023-12911-8 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10798932</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2917554098</sourcerecordid><originalsourceid>FETCH-LOGICAL-c426t-819e32947e5771e50efdfadb8036f56d0b380dada5d0118e82329bf55d90905c3</originalsourceid><addsrcrecordid>eNp9kU1vFSEUhomxsbfVP-DCTOLGhegBhhlYGdPUatKki-oameFwpZmBK8yY9N_L7a31Y-HqnISHl3N4CHnO4A0D6N8WAC4FBS4o45oxqh6RDWsFp9Cx9jHZAOsl7aVWx-SklBsAxlXXPSHHQvEWVMc35Ov1bVlwDmMz42KHNNUO4zZExBzitkm-OY8L5jTYsZbG1naLEUvjU27Q-zAGjEvDXws6rIuN6EKaml1Obh2XkOJTcuTtVPDZfT0lXz6cfz77SC-vLj6dvb-kY8u7hSqmUXDd9ij7nqEE9M5bNygQnZedg0EocNZZ6YAxhYpXevBSOg0a5ChOybtD7m4dZnRjHSrbyexymG2-NckG8_dJDN_MNv0wDHqttOA14dV9Qk7fVyyLmUMZcZrqUmktpn5xL2ULWlX05T_oTVpzrPvtqU6CkEJXih-oMadSMvqHaRiYvUFzMGiqQXNn0OyjX_y5x8OVX8oqIA5A2e0NYf799n9ifwJOTqfK</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2916503539</pqid></control><display><type>article</type><title>Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production</title><source>Springer Nature - SpringerLink Journals - Fully Open Access</source><source>Springer Link</source><creator>Lu, Ping ; Bai, Ruoxuan ; Gao, Ting ; Chen, Jiale ; Jiang, Ke ; Zhu, Yalun ; Lu, Ye ; Zhang, Shuting ; Xu, Fangxu ; Zhao, Hongxin</creator><creatorcontrib>Lu, Ping ; Bai, Ruoxuan ; Gao, Ting ; Chen, Jiale ; Jiang, Ke ; Zhu, Yalun ; Lu, Ye ; Zhang, Shuting ; Xu, Fangxu ; Zhao, Hongxin</creatorcontrib><description>2,3-Butanediol (2,3-BDO) is an important gateway molecule for many chemical derivatives. Currently, microbial production is gradually being recognized as a green and sustainable alternative to petrochemical synthesis, but the titer, yield, and productivity of microbial 2,3-BDO remain suboptimal. Here, we used systemic metabolic engineering strategies to debottleneck the 2,3-BDO production in
Enterobacter aerogenes
. Firstly, the pyruvate metabolic network was reconstructed by deleting genes for by-product synthesis to improve the flux toward 2,3-BDO synthesis, which resulted in a 90% increase of the product titer. Secondly, the 2,3-BDO productivity of the IAM1183-LPCT/D was increased by 55% due to the heterologous expression of DR1558 which boosted cell resistance to abiotic stress. Thirdly, carbon sources were optimized to further improve the yield of target products. The IAM1183-LPCT/D showed the highest titer of 2,3-BDO from sucrose, 20% higher than that from glucose, and the yield of 2,3-BDO reached 0.49 g/g. Finally, the titer of 2,3-BDO of IAM1183-LPCT/D in a 5-L fermenter reached 22.93 g/L, 85% higher than the wild-type strain, and the titer of by-products except ethanol was very low.
Key points
Deletion of five key genes in E. aerogenes improved 2,3-BDO production
The titer of 2,3-BDO was increased by 90% by regulating metabolic flux
Response regulator DR1558 was expressed to increase 2,3-BDO productivity
Graphical abstract</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-023-12911-8</identifier><identifier>PMID: 38240862</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aerogenes ; Bioenergy and Biofuels ; Biomedical and Life Sciences ; Bioreactors ; Biotechnology ; Butanediol ; Butylene Glycols - metabolism ; Byproducts ; Carbon sources ; Enterobacter aerogenes ; Enterobacter aerogenes - genetics ; Enterobacter aerogenes - metabolism ; Ethanol ; Fermentation ; Genes ; Life Sciences ; Metabolic engineering ; Metabolic Engineering - methods ; Metabolic flux ; Metabolic networks ; Metabolism ; Microbial Genetics and Genomics ; Microbiology ; Microorganisms ; Petrochemicals ; Productivity ; Pyruvic acid ; Sucrose ; Synthesis</subject><ispartof>Applied microbiology and biotechnology, 2024-12, Vol.108 (1), p.146-146, Article 146</ispartof><rights>The Author(s) 2024</rights><rights>2024. The Author(s).</rights><rights>The Author(s) 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c426t-819e32947e5771e50efdfadb8036f56d0b380dada5d0118e82329bf55d90905c3</cites><orcidid>0000-0001-5238-506X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38240862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Ping</creatorcontrib><creatorcontrib>Bai, Ruoxuan</creatorcontrib><creatorcontrib>Gao, Ting</creatorcontrib><creatorcontrib>Chen, Jiale</creatorcontrib><creatorcontrib>Jiang, Ke</creatorcontrib><creatorcontrib>Zhu, Yalun</creatorcontrib><creatorcontrib>Lu, Ye</creatorcontrib><creatorcontrib>Zhang, Shuting</creatorcontrib><creatorcontrib>Xu, Fangxu</creatorcontrib><creatorcontrib>Zhao, Hongxin</creatorcontrib><title>Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>2,3-Butanediol (2,3-BDO) is an important gateway molecule for many chemical derivatives. Currently, microbial production is gradually being recognized as a green and sustainable alternative to petrochemical synthesis, but the titer, yield, and productivity of microbial 2,3-BDO remain suboptimal. Here, we used systemic metabolic engineering strategies to debottleneck the 2,3-BDO production in
Enterobacter aerogenes
. Firstly, the pyruvate metabolic network was reconstructed by deleting genes for by-product synthesis to improve the flux toward 2,3-BDO synthesis, which resulted in a 90% increase of the product titer. Secondly, the 2,3-BDO productivity of the IAM1183-LPCT/D was increased by 55% due to the heterologous expression of DR1558 which boosted cell resistance to abiotic stress. Thirdly, carbon sources were optimized to further improve the yield of target products. The IAM1183-LPCT/D showed the highest titer of 2,3-BDO from sucrose, 20% higher than that from glucose, and the yield of 2,3-BDO reached 0.49 g/g. Finally, the titer of 2,3-BDO of IAM1183-LPCT/D in a 5-L fermenter reached 22.93 g/L, 85% higher than the wild-type strain, and the titer of by-products except ethanol was very low.
Key points
Deletion of five key genes in E. aerogenes improved 2,3-BDO production
The titer of 2,3-BDO was increased by 90% by regulating metabolic flux
Response regulator DR1558 was expressed to increase 2,3-BDO productivity
Graphical abstract</description><subject>Aerogenes</subject><subject>Bioenergy and Biofuels</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Butanediol</subject><subject>Butylene Glycols - metabolism</subject><subject>Byproducts</subject><subject>Carbon sources</subject><subject>Enterobacter aerogenes</subject><subject>Enterobacter aerogenes - genetics</subject><subject>Enterobacter aerogenes - metabolism</subject><subject>Ethanol</subject><subject>Fermentation</subject><subject>Genes</subject><subject>Life Sciences</subject><subject>Metabolic engineering</subject><subject>Metabolic Engineering - methods</subject><subject>Metabolic flux</subject><subject>Metabolic networks</subject><subject>Metabolism</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Petrochemicals</subject><subject>Productivity</subject><subject>Pyruvic acid</subject><subject>Sucrose</subject><subject>Synthesis</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kU1vFSEUhomxsbfVP-DCTOLGhegBhhlYGdPUatKki-oameFwpZmBK8yY9N_L7a31Y-HqnISHl3N4CHnO4A0D6N8WAC4FBS4o45oxqh6RDWsFp9Cx9jHZAOsl7aVWx-SklBsAxlXXPSHHQvEWVMc35Ov1bVlwDmMz42KHNNUO4zZExBzitkm-OY8L5jTYsZbG1naLEUvjU27Q-zAGjEvDXws6rIuN6EKaml1Obh2XkOJTcuTtVPDZfT0lXz6cfz77SC-vLj6dvb-kY8u7hSqmUXDd9ij7nqEE9M5bNygQnZedg0EocNZZ6YAxhYpXevBSOg0a5ChOybtD7m4dZnRjHSrbyexymG2-NckG8_dJDN_MNv0wDHqttOA14dV9Qk7fVyyLmUMZcZrqUmktpn5xL2ULWlX05T_oTVpzrPvtqU6CkEJXih-oMadSMvqHaRiYvUFzMGiqQXNn0OyjX_y5x8OVX8oqIA5A2e0NYf799n9ifwJOTqfK</recordid><startdate>20241201</startdate><enddate>20241201</enddate><creator>Lu, Ping</creator><creator>Bai, Ruoxuan</creator><creator>Gao, Ting</creator><creator>Chen, Jiale</creator><creator>Jiang, Ke</creator><creator>Zhu, Yalun</creator><creator>Lu, Ye</creator><creator>Zhang, Shuting</creator><creator>Xu, Fangxu</creator><creator>Zhao, Hongxin</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5238-506X</orcidid></search><sort><creationdate>20241201</creationdate><title>Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production</title><author>Lu, Ping ; Bai, Ruoxuan ; Gao, Ting ; Chen, Jiale ; Jiang, Ke ; Zhu, Yalun ; Lu, Ye ; Zhang, Shuting ; Xu, Fangxu ; Zhao, Hongxin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-819e32947e5771e50efdfadb8036f56d0b380dada5d0118e82329bf55d90905c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aerogenes</topic><topic>Bioenergy and Biofuels</topic><topic>Biomedical and Life Sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Butanediol</topic><topic>Butylene Glycols - metabolism</topic><topic>Byproducts</topic><topic>Carbon sources</topic><topic>Enterobacter aerogenes</topic><topic>Enterobacter aerogenes - genetics</topic><topic>Enterobacter aerogenes - metabolism</topic><topic>Ethanol</topic><topic>Fermentation</topic><topic>Genes</topic><topic>Life Sciences</topic><topic>Metabolic engineering</topic><topic>Metabolic Engineering - methods</topic><topic>Metabolic flux</topic><topic>Metabolic networks</topic><topic>Metabolism</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Petrochemicals</topic><topic>Productivity</topic><topic>Pyruvic acid</topic><topic>Sucrose</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Ping</creatorcontrib><creatorcontrib>Bai, Ruoxuan</creatorcontrib><creatorcontrib>Gao, Ting</creatorcontrib><creatorcontrib>Chen, Jiale</creatorcontrib><creatorcontrib>Jiang, Ke</creatorcontrib><creatorcontrib>Zhu, Yalun</creatorcontrib><creatorcontrib>Lu, Ye</creatorcontrib><creatorcontrib>Zhang, Shuting</creatorcontrib><creatorcontrib>Xu, Fangxu</creatorcontrib><creatorcontrib>Zhao, Hongxin</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Ping</au><au>Bai, Ruoxuan</au><au>Gao, Ting</au><au>Chen, Jiale</au><au>Jiang, Ke</au><au>Zhu, Yalun</au><au>Lu, Ye</au><au>Zhang, Shuting</au><au>Xu, Fangxu</au><au>Zhao, Hongxin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2024-12-01</date><risdate>2024</risdate><volume>108</volume><issue>1</issue><spage>146</spage><epage>146</epage><pages>146-146</pages><artnum>146</artnum><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>2,3-Butanediol (2,3-BDO) is an important gateway molecule for many chemical derivatives. Currently, microbial production is gradually being recognized as a green and sustainable alternative to petrochemical synthesis, but the titer, yield, and productivity of microbial 2,3-BDO remain suboptimal. Here, we used systemic metabolic engineering strategies to debottleneck the 2,3-BDO production in
Enterobacter aerogenes
. Firstly, the pyruvate metabolic network was reconstructed by deleting genes for by-product synthesis to improve the flux toward 2,3-BDO synthesis, which resulted in a 90% increase of the product titer. Secondly, the 2,3-BDO productivity of the IAM1183-LPCT/D was increased by 55% due to the heterologous expression of DR1558 which boosted cell resistance to abiotic stress. Thirdly, carbon sources were optimized to further improve the yield of target products. The IAM1183-LPCT/D showed the highest titer of 2,3-BDO from sucrose, 20% higher than that from glucose, and the yield of 2,3-BDO reached 0.49 g/g. Finally, the titer of 2,3-BDO of IAM1183-LPCT/D in a 5-L fermenter reached 22.93 g/L, 85% higher than the wild-type strain, and the titer of by-products except ethanol was very low.
Key points
Deletion of five key genes in E. aerogenes improved 2,3-BDO production
The titer of 2,3-BDO was increased by 90% by regulating metabolic flux
Response regulator DR1558 was expressed to increase 2,3-BDO productivity
Graphical abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38240862</pmid><doi>10.1007/s00253-023-12911-8</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-5238-506X</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2024-12, Vol.108 (1), p.146-146, Article 146 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10798932 |
| source | Springer Nature - SpringerLink Journals - Fully Open Access; Springer Link |
| subjects | Aerogenes Bioenergy and Biofuels Biomedical and Life Sciences Bioreactors Biotechnology Butanediol Butylene Glycols - metabolism Byproducts Carbon sources Enterobacter aerogenes Enterobacter aerogenes - genetics Enterobacter aerogenes - metabolism Ethanol Fermentation Genes Life Sciences Metabolic engineering Metabolic Engineering - methods Metabolic flux Metabolic networks Metabolism Microbial Genetics and Genomics Microbiology Microorganisms Petrochemicals Productivity Pyruvic acid Sucrose Synthesis |
| title | Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T05%3A04%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Systemic%20metabolic%20engineering%20of%20Enterobacter%20aerogenes%20for%20efficient%202,3-butanediol%20production&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=Lu,%20Ping&rft.date=2024-12-01&rft.volume=108&rft.issue=1&rft.spage=146&rft.epage=146&rft.pages=146-146&rft.artnum=146&rft.issn=0175-7598&rft.eissn=1432-0614&rft_id=info:doi/10.1007/s00253-023-12911-8&rft_dat=%3Cproquest_pubme%3E2917554098%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c426t-819e32947e5771e50efdfadb8036f56d0b380dada5d0118e82329bf55d90905c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2916503539&rft_id=info:pmid/38240862&rfr_iscdi=true |