Processing of stalled replication forks in Bacillus subtilis

• Published in: FEMS microbiology reviews 2024-01, Vol.48 (1)
• Main Authors: Carrasco, Begoña, Torres, Rubén, Moreno-Del Álamo, María, Ramos, Cristina, Ayora, Silvia, Alonso, Juan C
• Format: Article
• Language: English
• Subjects: Bacillus subtilis - genetics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; DNA - metabolism; DNA Replication - genetics; Escherichia coli Proteins - genetics; Review
• ISSN: 1574-6976; 0168-6445; 1574-6976
• DOI: 10.1093/femsre/fuad065

Accurate DNA replication and transcription elongation are crucial for preventing the accumulation of unreplicated DNA and genomic instability. Cells have evolved multiple mechanisms to deal with impaired replication fork progression, challenged by both intrinsic and extrinsic impediments. The bacterium Bacillus subtilis, which adopts multiple forms of differentiation and development, serves as an excellent model system for studying the pathways required to cope with replication stress to preserve genomic stability. This review focuses on the genetics, single molecule choreography, and biochemical properties of the proteins that act to circumvent the replicative arrest allowing the resumption of DNA synthesis. The RecA recombinase, its mediators (RecO, RecR, and RadA/Sms) and modulators (RecF, RecX, RarA, RecU, RecD2, and PcrA), repair licensing (DisA), fork remodelers (RuvAB, RecG, RecD2, RadA/Sms, and PriA), Holliday junction resolvase (RecU), nucleases (RnhC and DinG), and translesion synthesis DNA polymerases (PolY1 and PolY2) are key functions required to overcome a replication stress, provided that the fork does not collapse.