Nitrous oxide reduction by two partial denitrifying bacteria requires denitrification intermediates that cannot be respired

• Published in: Applied and environmental microbiology 2024-01, Vol.90 (1), p.e0174123-e0174123
• Main Authors: LaSarre, Breah, Morlen, Ryan, Neumann, Gina C, Harwood, Caroline S, McKinlay, James B
• Format: Article
• Language: English
• Subjects: Anaerobic respiration; Annotations; Bacteria; Bacteriology; Darkness; Denitrification; Denitrifying bacteria; Energy conversion; Enzymatic activity; Enzyme activity; Enzymes; Galactosidase; Gene expression; Genomes; Intermediates; Nitrates; Nitric oxide; Nitrogen dioxide; Nitrous oxide; Organic compounds; Physiology; Reductase; Reductases; Reduction; Respiration; β-Galactosidase
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/aem.01741-23

Denitrification is a form of microbial respiration wherein nitrate is converted via several nitrogen oxide intermediates into harmless dinitrogen gas. Partial denitrifying bacteria, which individually have some but not all denitrifying enzymes, can achieve complete denitrification as a community by cross-feeding nitrogen oxide intermediates. However, the last intermediate, nitrous oxide (N2O), is a potent greenhouse gas that often escapes, motivating efforts to understand and improve the efficiency of denitrification. Here, we found that at least some partial denitrifying N2O reducers can sense and respond to nitrogen oxide intermediates that they cannot otherwise use. The regulatory effects of nitrogen oxides on partial denitrifiers are thus an important consideration in understanding and applying denitrifying bacterial communities to combat greenhouse gas emissions.