Modulation of auxin-binding proteins in cell suspensions. I. Differential responses of carrot embryo cultures

This paper shows that the level of 2,4-dichlorophenoxyacetic acid (2,4-D) in the medium determines the level of auxin-binding proteins in the membranes of carrot, Daucus carota, cells grow in suspension. This induction takes slightly more than 2 hours to complete and can be elicited by natural as we...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1991-09, Vol.97 (1), p.60-64
Main Authors: LoSchiavo, F. (Universita della Basilicata, Potenza), Filippini, F, Cozzani, F, Vallone, D, Terzi, M
Format: Article
Language:English
Subjects:
2,4-D
AUXINAS
AUXINE
Auxins
Biological and medical sciences
Cell culture techniques
Cell growth
Cell lines
CULTIVO DE CELULAS
CULTURE DE CELLULE
DAUCUS CAROTA
DESARROLLO EMBRIONARIO
Development and Growth Regulation
DEVELOPPEMENT EMBRYONNAIRE
EMBRIONES VEGETALES
EMBRYON VEGETAL
Embryonic cells
Embryonic stem cells
Embryos
Fundamental and applied biological sciences. Psychology
MEMBRANAS CELULARES
MEMBRANE CELLULAIRE
Plant physiology and development
PROTEINAS
PROTEINE
Somatic cells
Stem cells
Tissue cultures, protoplasts
Totipotent stem cells
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Summary:This paper shows that the level of 2,4-dichlorophenoxyacetic acid (2,4-D) in the medium determines the level of auxin-binding proteins in the membranes of carrot, Daucus carota, cells grow in suspension. This induction takes slightly more than 2 hours to complete and can be elicited by natural as well as synthetic auxins. The auxin binding sites thus generated, which are pronase-sensitive, bind 2,4-D, indoleacetic acid, and naphthalene-acetic acid (NAA) equally well. However both alpha-and beta-NAA bind, whereas only alpha-NAA is effective in the inductive process. Cells committed to embryogeny (proembryogenic masses) do not respond to auxin, i.e. their level of auxin-binding proteins remains very low, and they do not seem to synthesize the hormone, as indicated by inhibitor studies. Sensitivity to, and production of, auxin, begins when the embryo becomes polarized, i.e. at post-globular stage
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.97.1.60