Expression of the Candida albicans gene ALS1 in Saccharomyces cerevisiae induces adherence to endothelial and epithelial cells

To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae. These transformed organisms were screened for adherence to endothelial cells, and a highly adher...

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Bibliographic Details
Published in:Infection and immunity 1998-04, Vol.66 (4), p.1783-1786
Main Authors: YUE FU, RIEG, G, FONZI, W. A, BELANGER, P. H, EDWARDS, J. E, FILLER, S. G
Format: Article
Language:English
Subjects:
Adhesiveness
Biological and medical sciences
Candida albicans - genetics
Endothelium, Vascular - microbiology
Epithelial Cells - microbiology
Fundamental and applied biological sciences. Psychology
Fungal and Parasitic Infections
Fungal Proteins - chemistry
Fungal Proteins - genetics
Genes, Fungal
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Humans
Microbiology
Mycology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - physiology
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Summary:To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae. These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1. These results indicate that ALS1 may encode a candidal adhesin.
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.66.4.1783-1786.1998