Genome editing in rice using CRISPR/Cas12i3

Summary The CRISPR/Cas type V‐I is a family of programmable nuclease systems that prefers a T‐rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome‐editing application of Cas12i3, a type V‐I family endonuclease, was characterized in rice. We developed a CRIP...

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Bibliographic Details
Published in:Plant biotechnology journal 2024-02, Vol.22 (2), p.379-385
Main Authors: Lv, Ping, Su, Fei, Chen, Fangyuan, Yan, Chunxue, Xia, Dandan, Sun, Hui, Li, Shanshan, Duan, Zhiqiang, Ma, Changle, Zhang, Hui, Wang, Mugui, Niu, Xiaomu, Zhu, Jian‐Kang, Zhang, Jinshan
Format: Article
Language:English
Subjects:
Arrays
Base editors
CRISPR
CRISPR/Cas12i3
DNA structural variations
Editing
Efficiency
Endonuclease
Gene expression
Genome editing
Genomes
Herbicides
Multiplex DR‐spacer Array
Mutation
Nuclease
Plant breeding
Rice
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Summary:Summary The CRISPR/Cas type V‐I is a family of programmable nuclease systems that prefers a T‐rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome‐editing application of Cas12i3, a type V‐I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3‐based Multiplex direct repeats (DR)‐spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide‐resistant rice plants using the base editors. These CRIPSR/Cas12i3‐based genome editing systems will facilitate precision molecular breeding in plants.
ISSN:1467-7644
1467-7652
1467-7652
DOI:10.1111/pbi.14192