Blood culture identification (BCID) performance in polymicrobial bacteremia

The rapid multiplex PCR (rmPCR)-based FilmArray® blood culture identification (BCID) assay reduces time from positive blood culture to organism identification. Polymicrobial bacteremia (PMB) is a known area of reduced diagnostic fidelity for BCID and remains incompletely characterized. All cases of...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2023-12, Vol.107 (4), p.116086-116086, Article 116086
Main Authors: Bradford, William S., Donnelley, Monica A., Fine, Jeffrey R., Crabtree, Scott J.
Format: Article
Language:English
Subjects:
Bacteremia
Bacteremia - diagnosis
BCID
Blood Culture - methods
FilmArray
Humans
Multiplex Polymerase Chain Reaction
Polymicrobial
Retrospective Studies
Time Factors
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Summary:The rapid multiplex PCR (rmPCR)-based FilmArray® blood culture identification (BCID) assay reduces time from positive blood culture to organism identification. Polymicrobial bacteremia (PMB) is a known area of reduced diagnostic fidelity for BCID and remains incompletely characterized. All cases of clinically confirmed PMB at a large academic single center from a 23-month period were evaluated in a retrospective cohort analysis. A total of 207 samples were identified and studied. Overall, 49.3% (N = 102) of polymicrobial cultures were incompletely identified by FilmArray® result. There were no significant between-group differences in comorbidity status, length of stay, mortality, or source between patients with PMB who had complete versus incomplete BCID identification. Our results suggest that rmPCR-based assays frequently miss organisms in PMB and should be interpreted accordingly.
ISSN:0732-8893
1879-0070
1879-0070
DOI:10.1016/j.diagmicrobio.2023.116086