Taxogenomic analysis of a novel yeast species isolated from soil, Pichia galeolata sp. nov

A novel budding yeast species was isolated from a soil sample collected in the United States of America. Phylogenetic analyses of multiple loci and phylogenomic analyses conclusively placed the species within the genus Pichia. Strain yHMH446 falls within a clade that includes Pichia norvegensis, Pic...

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Published in:Yeast (Chichester, England) England), 2023-12, Vol.40 (12), p.608-615
Main Authors: Opulente, Dana A., Langdon, Quinn K., Jarzyna, Martin, Buh, Kelly V., Haase, Max A. B., Groenewald, Marizeth, Hittinger, Chris Todd
Format: Article
Language:English
Subjects:
BASIC BIOLOGICAL SCIENCES
Carbon
Carbon sources
DNA, Fungal - genetics
genome sequence
Genomes
Genomic analysis
genotype‐phenotype map
Mycological Typing Techniques
New species
Nitrogen
Nitrogen sources
novel species
Nucleotide sequence
Phylogeny
Pichia
Pichia - genetics
Sequence Analysis, DNA
Soil
taxogenomics
yeast
Yeasts - genetics
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Summary:A novel budding yeast species was isolated from a soil sample collected in the United States of America. Phylogenetic analyses of multiple loci and phylogenomic analyses conclusively placed the species within the genus Pichia. Strain yHMH446 falls within a clade that includes Pichia norvegensis, Pichia pseudocactophila, Candida inconspicua, and Pichia cactophila. Whole genome sequence data were analyzed for the presence of genes known to be important for carbon and nitrogen metabolism, and the phenotypic data from the novel species were compared to all Pichia species with publicly available genomes. Across the genus, including the novel species candidate, we found that the inability to use many carbon and nitrogen sources correlated with the absence of metabolic genes. Based on these results, Pichia galeolata sp. nov. is proposed to accommodate yHMH446T (=NRRL Y‐64187 = CBS 16864). This study shows how integrated taxogenomic analysis can add mechanistic insight to species descriptions. The discovery, formal description, and taxogenomic analysis of a novel species of Pichia revealed correlations between genome content and traditional physiological assays. Take‐away The novel species Pichia galeolata is discovered and formally described. The genome sequence of P. galeolata is reported. Taxogenomic analysis shows how genome content correlates with traditional physiological assays.
ISSN:0749-503X
1097-0061
1097-0061
DOI:10.1002/yea.3905