Applications of Charge Detection Mass Spectrometry in Molecular Biology and Biotechnology

Charge detection mass spectrometry (CDMS) is a single-particle technique where the masses of individual ions are determined from simultaneous measurement of their mass-to-charge ratio (m/z) and charge. Masses are determined for thousands of individual ions, and then the results are binned to give a...

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Bibliographic Details
Published in:Chemical reviews 2022-04, Vol.122 (8), p.7415-7441
Main Author: Jarrold, Martin F
Format: Article
Language:English
Subjects:
Biotechnology
Exosomes
Gene therapy
Ions
Mass spectrometry
Mass Spectrometry - methods
Molecular Biology
Proteins
Scientific imaging
Spectroscopy
Vaccines
Viruses
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Summary:Charge detection mass spectrometry (CDMS) is a single-particle technique where the masses of individual ions are determined from simultaneous measurement of their mass-to-charge ratio (m/z) and charge. Masses are determined for thousands of individual ions, and then the results are binned to give a mass spectrum. Using this approach, accurate mass distributions can be measured for heterogeneous and high-molecular-weight samples that are usually not amenable to analysis by conventional mass spectrometry. Recent applications include heavily glycosylated proteins, protein complexes, protein aggregates such as amyloid fibers, infectious viruses, gene therapies, vaccines, and vesicles such as exosomes.
ISSN:0009-2665
1520-6890
DOI:10.1021/acs.chemrev.1c00377