Vector development for the expression of foreign proteins in the vaccine strain Brucella abortus S19

A vector for the expression of foreign antigens in the vaccine strain Brucella abortus S19 was developed by using a DNA fragment containing the regulatory sequences and the signal peptide of the Brucella bcsp31 gene. This fragment was cloned in broad-host-range plasmid pBBR4MCS, resulting in plasmid...

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Bibliographic Details
Published in:Infection and immunity 1998-08, Vol.66 (8), p.3862-3866
Main Authors: COMERCI, D. J, POLLEVICK, G. D, VIGLIOCCO, A. M, FRASCH, A. C. C, UGALDE, R. A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animal bacterial diseases
Animals
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Antigens, Protozoan - genetics
Antigens, Protozoan - immunology
Bacterial diseases
Bacterial Vaccines - genetics
Bacterial Vaccines - immunology
Bacteriology
Base Sequence
Biological and medical sciences
Brucella abortus
Brucella abortus - genetics
Brucella abortus - immunology
Brucella abortus - physiology
Brucellosis - microbiology
Cloning, Molecular
DNA, Bacterial
Female
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes, Reporter
Genetic Vectors
Infectious diseases
Medical sciences
Mice
Mice, Inbred BALB C
Microbial Immunity and Vaccines
Microbiology
Molecular Sequence Data
Rabbits
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Trypanosoma cruzi
Trypanosoma cruzi - genetics
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:A vector for the expression of foreign antigens in the vaccine strain Brucella abortus S19 was developed by using a DNA fragment containing the regulatory sequences and the signal peptide of the Brucella bcsp31 gene. This fragment was cloned in broad-host-range plasmid pBBR4MCS, resulting in plasmid pBEV. As a reporter protein, a repetitive antigen of Trypanosoma cruzi was used. The recombinant fusion protein is stably expressed and secreted into the Brucella periplasmic space, inducing a good antibody response against the T. cruzi antigen. The expression of the repetitive antigen in Brucella neither altered its growth pattern nor generated a toxic or lethal effect during experimental infection. The application of this strategy for the generation of live recombinant vaccines and the tagging of B. abortus S19 vaccine is discussed. This is the first time that a recombinant protein has been expressed in the periplasm of brucellae.
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.66.8.3862-3866.1998