Deletion of the central proline-rich repeat domain results in altered antigenicity and lack of surface expression of the Streptococcus mutans P1 adhesin molecule

• Published in: Infection and immunity 1998-09, Vol.66 (9), p.4274-4282
• Main Authors: BRADY, L. J, CVITKOVITCH, D. G, GERIC, C. M, ADDISON, M. N, JOYCE, J. C, CROWLEY, P. J, BLEIWEIS, A. S
• Format: Article
• Language: English
• Subjects: Adhesins, Bacterial - genetics; Adhesins, Bacterial - immunology; Animals; Antibodies, Monoclonal - immunology; Antigens, Bacterial - genetics; Antigens, Bacterial - immunology; Bacterial Infections; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacteriology; Biological and medical sciences; Blotting, Western; Epitopes, B-Lymphocyte - genetics; Epitopes, B-Lymphocyte - immunology; Escherichia coli; Fundamental and applied biological sciences. Psychology; Membrane Glycoproteins; Mice; Microbiology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Plasmids; Proline - genetics; Proline - immunology; Rabbits; Repetitive Sequences, Nucleic Acid; RNA, Messenger; Sequence Deletion; Streptococcus mutans; Streptococcus mutans - genetics; Streptococcus mutans - immunology; Transformation, Bacterial
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/iai.66.9.4274-4282.1998

Members of the family of surface adhesins of oral streptococci, including P1 of Streptococcus mutans, contain two highly conserved repeat domains, one rich in alanine (A region) and the other rich in proline (P region). To assess the contribution of the P region to the biological properties of P1, an internal deletion in spaP was engineered. In addition, the P region was subcloned and expressed as a fusion partner with the maltose binding protein of Escherichia coli and liberated by digestion with factor Xa. Results of Western blot experiments in which recombinant polypeptides were probed with a panel of 11 monoclonal antibodies indicated that the P region is a necessary component of conformational epitopes within the central portion of P1. Antibodies reactive with the P region were detected in a polyclonal rabbit antiserum generated against whole S. mutans cells but not in two rabbit antisera generated against purified P1 (Mr approximately 185,000), suggesting that this domain is immunogenic on the surface of intact bacteria but not as part of a soluble full-length molecule. Finally, transformation of a spaP-negative mutant with a shuttle vector containing an internally deleted spaP lacking P-region DNA resulted in a complete absence of surface-localized P1 and substantially less P1 in sonicated cells compared to the case for the mutant complemented with the full-length gene. These results suggest that the P region is an integral component contributing to the conformation of the central region of P1 and indicate that its presence is necessary for surface expression of the molecule on S. mutans.