Luteinizing hormone stimulates ingression of mural granulosa cells within the mouse preovulatory follicle

Luteinizing hormone (LH) induces ovulation by acting on its receptors in the mural granulosa cells that surround a mammalian oocyte in an ovarian follicle. However, much remains unknown about how activation of the LH receptor modifies the structure of the follicle such that the oocyte is released an...

Full description

Saved in:
Bibliographic Details
Published in:Biology of reproduction 2024-02, Vol.110 (2), p.288-299
Main Authors: Owen, Corie M., Jaffe, Laurinda A.
Format: Article
Language:English
Subjects:
Animals
Basal lamina
Corpus luteum
Female
Follicles
granulosa cell
Granulosa cells
Granulosa Cells - metabolism
ingression
Invaginations
Luteinizing hormone
Luteinizing Hormone - metabolism
luteinizing hormone receptor
Mammals - metabolism
Mice
migration
ovarian follicle
Ovarian Follicle - metabolism
Ovulation
Ovulation - physiology
Receptors, LH - metabolism
RESEARCH ARTICLE
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Luteinizing hormone (LH) induces ovulation by acting on its receptors in the mural granulosa cells that surround a mammalian oocyte in an ovarian follicle. However, much remains unknown about how activation of the LH receptor modifies the structure of the follicle such that the oocyte is released and the follicle remnants are transformed into the corpus luteum. The present study shows that the preovulatory surge of LH stimulates LH receptor-expressing granulosa cells, initially located almost entirely in the outer layers of the mural granulosa, to rapidly extend inwards, intercalating between other cells. The cellular ingression begins within 30 min of the peak of the LH surge, and the proportion of LH receptor-expressing cell bodies in the inner half of the mural granulosa layer increases until the time of ovulation, which occurs at about 10 h after the LH peak. During this time, many of the initially flask-shaped cells appear to detach from the basal lamina, acquiring a rounder shape with multiple filipodia. Starting at about 4 h after the LH peak, the mural granulosa layer at the apical surface of the follicle where ovulation will occur begins to thin, and the basolateral surface develops invaginations and constrictions. Our findings raise the question of whether LH stimulation of granulosa cell ingression may contribute to these changes in the follicular structure that enable ovulation. Summary Sentence LH-induced ingression of LH receptor-expressing cells within the mural granulosa layer of the ovarian follicle is a new component in the complex sequence of structural changes that lead to ovulation. Graphical Abstract
ISSN:0006-3363
1529-7268
1529-7268
DOI:10.1093/biolre/ioad142