Retroviruses and yeast retrotransposons use overlapping sets of host genes

A collection of 4457 Saccharomyces cerevisiae mutants deleted for nonessential genes was screened for mutants with increased or decreased mobilization of the gypsylike retroelement Ty3. Of these, 64 exhibited increased and 66 decreased Ty3 transposition compared with the parental strain. Genes ident...

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Bibliographic Details
Published in:Genome research 2005-05, Vol.15 (5), p.641-654
Main Authors: Irwin, Becky, Aye, Michael, Baldi, Pierre, Beliakova-Bethell, Nadejda, Cheng, Henry, Dou, Yimeng, Liou, Willy, Sandmeyer, Suzanne
Format: Article
Language:English
Subjects:
Blotting, Southern
Chromatin - genetics
Computational Biology - methods
DNA Mutational Analysis
DNA, Complementary - genetics
Fungal Proteins - genetics
Gene Expression Regulation, Fungal - genetics
Integration Host Factors - genetics
Mutation - genetics
Nuclear Pore - genetics
Nuclear Pore - metabolism
Retroelements - genetics
Saccharomyces cerevisiae - genetics
Schizosaccharomyces - genetics
Transcription, Genetic - genetics
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Summary:A collection of 4457 Saccharomyces cerevisiae mutants deleted for nonessential genes was screened for mutants with increased or decreased mobilization of the gypsylike retroelement Ty3. Of these, 64 exhibited increased and 66 decreased Ty3 transposition compared with the parental strain. Genes identified in this screen were grouped according to function by using GOnet software developed as part of this study. Gene clusters were related to chromatin and transcript elongation, translation and cytoplasmic RNA processing, vesicular trafficking, nuclear transport, and DNA maintenance. Sixty-six of the mutants were tested for Ty3 proteins and cDNA. Ty3 cDNA and transposition were increased in mutants affected in nuclear pore biogenesis and in a subset of mutants lacking proteins that interact physically or genetically with a replication clamp loader. Our results suggest that nuclear entry is linked mechanistically to Ty3 cDNA synthesis but that host replication factors antagonize Ty3 replication. Some of the factors we identified have been previously shown to affect Ty1 transposition and others to affect retroviral budding. Host factors, such as these, shared by distantly related Ty retroelements and retroviruses are novel candidates for antiviral targets.
ISSN:1088-9051
1549-5469
DOI:10.1101/gr.3739005