Disruption of the bacterial OLE RNP complex impairs growth on alternative carbon sources

Abstract Ornate, large, extremophilic (OLE) RNAs comprise a class of large noncoding RNAs in bacteria whose members form a membrane-associated ribonucleoprotein (RNP) complex. This complex facilitates cellular adaptation to diverse stresses such as exposure to cold, short-chain alcohols, and elevate...

Full description

Saved in:
Bibliographic Details
Published in:PNAS nexus 2024-02, Vol.3 (2), p.pgae075-pgae075
Main Authors: Lyon, Seth E, Wencker, Freya D R, Fernando, Chrishan M, Harris, Kimberly A, Breaker, Ronald R
Format: Article
Language:English
Subjects:
Bacteria
Biological, Health, and Medical Sciences
Carbon
Genetic aspects
Physiological aspects
Ribonucleoproteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Ornate, large, extremophilic (OLE) RNAs comprise a class of large noncoding RNAs in bacteria whose members form a membrane-associated ribonucleoprotein (RNP) complex. This complex facilitates cellular adaptation to diverse stresses such as exposure to cold, short-chain alcohols, and elevated Mg2+ concentrations. Here, we report additional phenotypes exhibited by Halalkalibacterium halodurans (formerly called Bacillus halodurans) strains lacking functional OLE RNP complexes. Genetic disruption of the complex causes restricted growth compared to wild-type cells when cultured in minimal media (MM) wherein glucose is replaced with alternative carbon/energy sources. Genetic suppressor selections conducted in glutamate MM yielded isolates that carry mutations in or near genes relevant to Mn2+ homeostasis (ykoY and mntB), phosphate homeostasis (phoR), and putative multidrug resistance (bmrCD). These functional links between OLE RNA, carbon/energy management, and other fundamental processes including protein secretion are consistent with the hypothesis that the OLE RNP complex is a major contributor to cellular adaptation to unfavorable growth conditions.
ISSN:2752-6542
2752-6542
DOI:10.1093/pnasnexus/pgae075