Correlation of miR-150, hsa-let-7e, and miR- 146a and gene expression of IL-6, IL-8, IP-10 , and MIP-1β during dengue virus infection

• Published in: Narra J 2021-04, Vol.1 (1), p.e31-e31
• Main Authors: Masyeni, Sri, Kuntaman, Kuntaman, Aryati, Aryati, Sofro, Muchlis Achsan Udji, Hadi, Usman, Mastutik, Gondo, Purnomo, Windu, Santosa, Agus, Yohan, Benediktus, Nelwan, Erni Juwita, Sasmono, R Tedjo
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 2807-2618; 2807-2618
• DOI: 10.52225/narraj.v1i1.31

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR- 150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines ( , and ) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p