Subcellular Localization of Glycosyl Transferases Involved in Glycoprotein Biosynthesis in the Cotyledons of Pisum sativum L. 1

Subcellular membrane fractions from 21-day-old pea ( Pisum sativum ) cotyledons that have associated UDP-N-acetylglucosamine N-acetylglucosaminyl transferase and GDP-mannose mannosyl transferase activities have been isolated and identified. The rough endoplasmic reticulum (RER) is the principal loca...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1978-03, Vol.61 (3), p.451-459
Main Authors: Nagahashi, Jerry, Beevers, Leonard
Format: Article
Language:English
Online Access:Get full text
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Summary:Subcellular membrane fractions from 21-day-old pea ( Pisum sativum ) cotyledons that have associated UDP-N-acetylglucosamine N-acetylglucosaminyl transferase and GDP-mannose mannosyl transferase activities have been isolated and identified. The rough endoplasmic reticulum (RER) is the principal location of glycosyl transferases involved in the assembly of lipid-linked sugar intermediates and glycoproteins. Antimycin A-insensitive NADH-cytochrome c reductase activity was used to identify RER at a density of 1.165 g/cc in sucrose gradients. The high proportion of RER in this fraction was confirmed by electron microscopy. Other mannosyl transferases are found at a density of 1.123 g/cc and 1.201 g/cc but these glycosyl transferases do not appear to be involved with the formation of lipid-linked sugar intermediates utilized in glycoprotein biosynthesis.
ISSN:0032-0889
1532-2548