Shuangshi Tonglin Capsule treats benign prostatic hyperplasia through the ROS/NLRP3 signaling pathway

• Published in: International urology and nephrology 2024-04, Vol.56 (4), p.1259-1271
• Main Authors: Wang, Ziqiang, Mao, Qian, Yuan, Yong, Wang, Chuan, Wei, Hao
• Format: Article
• Language: English
• Subjects: Animal models; Caspase-1; Castration; Collagen; CYBB protein; Enzyme-linked immunosorbent assay; Genital diseases; Hyperplasia; IL-1β; Immunohistochemistry; Inflammation; Medicine; Medicine & Public Health; Nephrology; NOX4 protein; Oral administration; Prostate; Sex hormones; Signal transduction; Tumor necrosis factor-α; Urology; Urology - Original Paper; Vesicles; Western blotting
• ISSN: 1573-2584; 0301-1623; 1573-2584
• DOI: 10.1007/s11255-023-03874-w

Objective To explore the effects of the SSTL on BPH and clarify the therapeutic mechanisms. Methods Animal model of BPH was established by castration and subcutaneous injection of TP into SD rats; rats were orally administered SSTL for 28 days while modeling. Detection of PI, LI and RI in rats, to observe histopathological changes and collagen deposition in the prostate tissue. Detects levels of sex hormones and inflammatory factors in serum and tissues of rats, the test kit detects levels of lipid peroxides and antioxidants in serum and tissues. Fluorescent staining analysis of tissue ROS; the expression of NLRP3 inflammatory vesicles was observed by immunohistochemistry; Western blotting detected the expression of NOX4, NOX2, NLRP3 inflammatory vesicles, ASC, Cleaved Caspase-1, Caspase-1, IL-1β. Results After SSTL capsule treatment, the PI and RI of the rats decrease. HE and Masson staining showed that SSTL ameliorated the pathological damage and reduced collagen deposition in the prostate tissue of BPH rats; ELISA results showed that SSTL was able to reduce T, DHT, TNF-α, IL-1β levels in BPH rats. The test kit showed that SSTL made the levels of MDA, CAT and GSH-Px in the serum and prostate tissue of rats and increased the activity of SOD. The results of ROS fluorescence showed that the ROS level was reduced in SSTL group; Western blotting showed that SSTL could cause down-regulation of NOX4, NOX2, NLRP3, ASC, Cleaved Caspase-1, IL-1β protein expression. Conclusion SSTL can reduce the PI and RI in BPH rats, it can also inhibit the level of sex hormones and inflammatory factors in BPH rats, which thereby reducing the histopathological damage of prostate gland in BPH rats, and can treat BPH in rats through ROS/NLRP3 pathway.