Establishment and application of a new 4/6 infarct nephrectomy rat model for moderate chronic kidney disease

• Published in: Acta Cirúrgica Brasileira 2024-01, Vol.39
• Main Authors: Sugai, Kazuhisa, Hirano, Momoko, Oda, Asahi, Fujisawa, Masahiko, Shono, Saori, Ishioka, Katsumi, Tamura, Tomoyoshi, Katsumata, Yoshinori, Sano, Motoaki, Kobayashi, Eiji, Hakamata, Yoji
• Format: Article
• Language: English
• Subjects: Chronic; Microsurgery; Nephrectomy; Original; Rats; Renal Insufficiency
• ISSN: 0102-8650; 1678-2674
• DOI: 10.1590/acb391324

ABSTRACT Purpose: To develop a new 4/6 infarct nephrectomy (INx) model rat mimicking moderate chronic kidney disease (CKD) and to evaluate its application. Methods: We modified the conventional 5/6 INx rat model to create the 4/6 INx model by ligating the renal artery branch to induce infarction of one-third of the left kidney after right kidney removal and compared biochemically and histologically both models. To demonstrate the application of the 4/6 INx model, the effects of a supplementary compound containing calcium carbonate, chitosan, palm shell activated charcoal etc., that is effective for both CKD and its complications, were compared between both models. Results: Impairment of renal function in the 4/6 INx group was significantly more moderate than in the 5/6 INx group (P < 0.05). The 4/6 INx group showed less histological damage in kidney than in the 5/6 INx group. The supplementary compound did not improve CKD in the 5/6 INx group, but ameliorated elevation of blood urea nitrogen in the 4/6 INx group. Conclusions: We developed the 4/6 INx model, which is more moderate than the conventional 5/6 INx model. This model could potentially demonstrate the effectiveness of drugs and supplements intended to prevent CKD and its progression.