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Human transferrin receptor can mediate SARS-CoV-2 infection
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been detected in almost all organs of coronavirus disease-19 patients, although some organs do not express angiotensin-converting enzyme-2 (ACE2), a known receptor of SARS-CoV-2, implying the presence of alternative receptors...
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Published in: | Proceedings of the National Academy of Sciences - PNAS 2024-03, Vol.121 (10), p.e2317026121-e2317026121 |
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creator | Liao, Zhiyi Wang, Chaoming Tang, Xiaopeng Yang, Mengli Duan, Zilei Liu, Lei Lu, Shuaiyao Ma, Lei Cheng, Ruomei Wang, Gan Liu, Hongqi Yang, Shuo Xu, Jingwen Tadese, Dawit Adisu Mwangi, James Kamau, Peter Muiruri Zhang, Zhiye Yang, Lian Liao, Guoyang Zhao, Xudong Peng, Xiaozhong Lai, Ren |
description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been detected in almost all organs of coronavirus disease-19 patients, although some organs do not express angiotensin-converting enzyme-2 (ACE2), a known receptor of SARS-CoV-2, implying the presence of alternative receptors and/or co-receptors. Here, we show that the ubiquitously distributed human transferrin receptor (TfR), which binds to diferric transferrin to traffic between membrane and endosome for the iron delivery cycle, can ACE2-independently mediate SARS-CoV-2 infection. Human, not mouse TfR, interacts with Spike protein with a high affinity (K
~2.95 nM) to mediate SARS-CoV-2 endocytosis. TfR knock-down (TfR-deficiency is lethal) and overexpression inhibit and promote SARS-CoV-2 infection, respectively. Humanized TfR expression enables SARS-CoV-2 infection in baby hamster kidney cells and C57 mice, which are known to be insusceptible to the virus infection. Soluble TfR, Tf, designed peptides blocking TfR-Spike interaction and anti-TfR antibody show significant anti-COVID-19 effects in cell and monkey models. Collectively, this report indicates that TfR is a receptor/co-receptor of SARS-CoV-2 mediating SARS-CoV-2 entry and infectivity by likely using the TfR trafficking pathway. |
doi_str_mv | 10.1073/pnas.2317026121 |
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~2.95 nM) to mediate SARS-CoV-2 endocytosis. TfR knock-down (TfR-deficiency is lethal) and overexpression inhibit and promote SARS-CoV-2 infection, respectively. Humanized TfR expression enables SARS-CoV-2 infection in baby hamster kidney cells and C57 mice, which are known to be insusceptible to the virus infection. Soluble TfR, Tf, designed peptides blocking TfR-Spike interaction and anti-TfR antibody show significant anti-COVID-19 effects in cell and monkey models. Collectively, this report indicates that TfR is a receptor/co-receptor of SARS-CoV-2 mediating SARS-CoV-2 entry and infectivity by likely using the TfR trafficking pathway.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.2317026121</identifier><identifier>PMID: 38408250</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>ACE2 ; Angiotensin ; Angiotensin-converting enzyme 2 ; Angiotensin-Converting Enzyme 2 - genetics ; Angiotensin-Converting Enzyme 2 - metabolism ; Animals ; Antibodies ; Biological Sciences ; Cell culture ; Coronaviruses ; COVID-19 ; Endocytosis ; Endosomes ; Humans ; Infections ; Infectivity ; Membrane trafficking ; Mice ; Organs ; Peptides ; Peptidyl-dipeptidase A ; Peptidyl-Dipeptidase A - metabolism ; Protein Binding ; Protein transport ; Receptors ; Receptors, Transferrin - genetics ; Receptors, Transferrin - metabolism ; Respiratory diseases ; SARS-CoV-2 - metabolism ; Severe acute respiratory syndrome coronavirus 2 ; Spike Glycoprotein, Coronavirus - metabolism ; Spike protein ; Transferrin ; Transferrins ; Viral diseases</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2024-03, Vol.121 (10), p.e2317026121-e2317026121</ispartof><rights>Copyright National Academy of Sciences Mar 5, 2024</rights><rights>Copyright © 2024 the Author(s). Published by PNAS. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-cb3e9e208a19bfb1de220379d6355026c16619b1daa4a3b8bf1b9a6afa14a2183</citedby><cites>FETCH-LOGICAL-c422t-cb3e9e208a19bfb1de220379d6355026c16619b1daa4a3b8bf1b9a6afa14a2183</cites><orcidid>0000-0002-6266-8503 ; 0000-0002-3123-2336 ; 0000-0002-9592-9554 ; 0000-0003-0293-2760 ; 0000-0003-1998-9763 ; 0000-0002-3262-9685 ; 0000-0002-8564-0247 ; 0000-0002-0580-2964</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927525/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10927525/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38408250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liao, Zhiyi</creatorcontrib><creatorcontrib>Wang, Chaoming</creatorcontrib><creatorcontrib>Tang, Xiaopeng</creatorcontrib><creatorcontrib>Yang, Mengli</creatorcontrib><creatorcontrib>Duan, Zilei</creatorcontrib><creatorcontrib>Liu, Lei</creatorcontrib><creatorcontrib>Lu, Shuaiyao</creatorcontrib><creatorcontrib>Ma, Lei</creatorcontrib><creatorcontrib>Cheng, Ruomei</creatorcontrib><creatorcontrib>Wang, Gan</creatorcontrib><creatorcontrib>Liu, Hongqi</creatorcontrib><creatorcontrib>Yang, Shuo</creatorcontrib><creatorcontrib>Xu, Jingwen</creatorcontrib><creatorcontrib>Tadese, Dawit Adisu</creatorcontrib><creatorcontrib>Mwangi, James</creatorcontrib><creatorcontrib>Kamau, Peter Muiruri</creatorcontrib><creatorcontrib>Zhang, Zhiye</creatorcontrib><creatorcontrib>Yang, Lian</creatorcontrib><creatorcontrib>Liao, Guoyang</creatorcontrib><creatorcontrib>Zhao, Xudong</creatorcontrib><creatorcontrib>Peng, Xiaozhong</creatorcontrib><creatorcontrib>Lai, Ren</creatorcontrib><title>Human transferrin receptor can mediate SARS-CoV-2 infection</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been detected in almost all organs of coronavirus disease-19 patients, although some organs do not express angiotensin-converting enzyme-2 (ACE2), a known receptor of SARS-CoV-2, implying the presence of alternative receptors and/or co-receptors. Here, we show that the ubiquitously distributed human transferrin receptor (TfR), which binds to diferric transferrin to traffic between membrane and endosome for the iron delivery cycle, can ACE2-independently mediate SARS-CoV-2 infection. Human, not mouse TfR, interacts with Spike protein with a high affinity (K
~2.95 nM) to mediate SARS-CoV-2 endocytosis. TfR knock-down (TfR-deficiency is lethal) and overexpression inhibit and promote SARS-CoV-2 infection, respectively. Humanized TfR expression enables SARS-CoV-2 infection in baby hamster kidney cells and C57 mice, which are known to be insusceptible to the virus infection. Soluble TfR, Tf, designed peptides blocking TfR-Spike interaction and anti-TfR antibody show significant anti-COVID-19 effects in cell and monkey models. Collectively, this report indicates that TfR is a receptor/co-receptor of SARS-CoV-2 mediating SARS-CoV-2 entry and infectivity by likely using the TfR trafficking pathway.</description><subject>ACE2</subject><subject>Angiotensin</subject><subject>Angiotensin-converting enzyme 2</subject><subject>Angiotensin-Converting Enzyme 2 - genetics</subject><subject>Angiotensin-Converting Enzyme 2 - metabolism</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Biological Sciences</subject><subject>Cell culture</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>Endocytosis</subject><subject>Endosomes</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectivity</subject><subject>Membrane trafficking</subject><subject>Mice</subject><subject>Organs</subject><subject>Peptides</subject><subject>Peptidyl-dipeptidase A</subject><subject>Peptidyl-Dipeptidase A - metabolism</subject><subject>Protein Binding</subject><subject>Protein transport</subject><subject>Receptors</subject><subject>Receptors, Transferrin - genetics</subject><subject>Receptors, Transferrin - metabolism</subject><subject>Respiratory diseases</subject><subject>SARS-CoV-2 - metabolism</subject><subject>Severe acute respiratory syndrome coronavirus 2</subject><subject>Spike Glycoprotein, Coronavirus - metabolism</subject><subject>Spike protein</subject><subject>Transferrin</subject><subject>Transferrins</subject><subject>Viral diseases</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpdkc1LAzEQxYMotlbP3mTBi5etmUn2Cw9SilqhIFj1GrLZrG5pk5rsCv73prTWj9Mc3m8eb-YRcgp0CDRjlysj_RAZZBRTQNgjfaAFxCkv6D7pU4pZnHPkPXLk_ZxSWiQ5PSQ9lnOaY0L75GrSLaWJWieNr7VzjYmcVnrVWhepICx11chWR7PR4ywe25cYo8bUWrWNNcfkoJYLr0-2c0Ceb2-expN4-nB3Px5NY8UR21iVTBcaaS6hKOsSKo1IWVZUKUuSkFtBmgYFKim5ZGVe1lAWMpW1BC4RcjYg1xvfVVeGPEqbEHchVq5ZSvcprGzEX8U0b-LVfojwC8wSTILDxdbB2fdO-1YsG6_0YiGNtp0XWDDkLEkhC-j5P3RuO2fCfYFKeCAhXxtebijlrPdO17s0QMW6GbFuRvw0EzbOfh-x47-rYF9SOokP</recordid><startdate>20240305</startdate><enddate>20240305</enddate><creator>Liao, Zhiyi</creator><creator>Wang, Chaoming</creator><creator>Tang, Xiaopeng</creator><creator>Yang, Mengli</creator><creator>Duan, Zilei</creator><creator>Liu, Lei</creator><creator>Lu, Shuaiyao</creator><creator>Ma, Lei</creator><creator>Cheng, Ruomei</creator><creator>Wang, Gan</creator><creator>Liu, Hongqi</creator><creator>Yang, Shuo</creator><creator>Xu, Jingwen</creator><creator>Tadese, Dawit Adisu</creator><creator>Mwangi, James</creator><creator>Kamau, Peter Muiruri</creator><creator>Zhang, Zhiye</creator><creator>Yang, Lian</creator><creator>Liao, Guoyang</creator><creator>Zhao, Xudong</creator><creator>Peng, Xiaozhong</creator><creator>Lai, Ren</creator><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6266-8503</orcidid><orcidid>https://orcid.org/0000-0002-3123-2336</orcidid><orcidid>https://orcid.org/0000-0002-9592-9554</orcidid><orcidid>https://orcid.org/0000-0003-0293-2760</orcidid><orcidid>https://orcid.org/0000-0003-1998-9763</orcidid><orcidid>https://orcid.org/0000-0002-3262-9685</orcidid><orcidid>https://orcid.org/0000-0002-8564-0247</orcidid><orcidid>https://orcid.org/0000-0002-0580-2964</orcidid></search><sort><creationdate>20240305</creationdate><title>Human transferrin receptor can mediate SARS-CoV-2 infection</title><author>Liao, Zhiyi ; Wang, Chaoming ; Tang, Xiaopeng ; Yang, Mengli ; Duan, Zilei ; Liu, Lei ; Lu, Shuaiyao ; Ma, Lei ; Cheng, Ruomei ; Wang, Gan ; Liu, Hongqi ; Yang, Shuo ; Xu, Jingwen ; Tadese, Dawit Adisu ; Mwangi, James ; Kamau, Peter Muiruri ; Zhang, Zhiye ; Yang, Lian ; Liao, Guoyang ; Zhao, Xudong ; Peng, Xiaozhong ; Lai, Ren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-cb3e9e208a19bfb1de220379d6355026c16619b1daa4a3b8bf1b9a6afa14a2183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>ACE2</topic><topic>Angiotensin</topic><topic>Angiotensin-converting enzyme 2</topic><topic>Angiotensin-Converting Enzyme 2 - 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Here, we show that the ubiquitously distributed human transferrin receptor (TfR), which binds to diferric transferrin to traffic between membrane and endosome for the iron delivery cycle, can ACE2-independently mediate SARS-CoV-2 infection. Human, not mouse TfR, interacts with Spike protein with a high affinity (K
~2.95 nM) to mediate SARS-CoV-2 endocytosis. TfR knock-down (TfR-deficiency is lethal) and overexpression inhibit and promote SARS-CoV-2 infection, respectively. Humanized TfR expression enables SARS-CoV-2 infection in baby hamster kidney cells and C57 mice, which are known to be insusceptible to the virus infection. Soluble TfR, Tf, designed peptides blocking TfR-Spike interaction and anti-TfR antibody show significant anti-COVID-19 effects in cell and monkey models. Collectively, this report indicates that TfR is a receptor/co-receptor of SARS-CoV-2 mediating SARS-CoV-2 entry and infectivity by likely using the TfR trafficking pathway.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>38408250</pmid><doi>10.1073/pnas.2317026121</doi><orcidid>https://orcid.org/0000-0002-6266-8503</orcidid><orcidid>https://orcid.org/0000-0002-3123-2336</orcidid><orcidid>https://orcid.org/0000-0002-9592-9554</orcidid><orcidid>https://orcid.org/0000-0003-0293-2760</orcidid><orcidid>https://orcid.org/0000-0003-1998-9763</orcidid><orcidid>https://orcid.org/0000-0002-3262-9685</orcidid><orcidid>https://orcid.org/0000-0002-8564-0247</orcidid><orcidid>https://orcid.org/0000-0002-0580-2964</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | ACE2 Angiotensin Angiotensin-converting enzyme 2 Angiotensin-Converting Enzyme 2 - genetics Angiotensin-Converting Enzyme 2 - metabolism Animals Antibodies Biological Sciences Cell culture Coronaviruses COVID-19 Endocytosis Endosomes Humans Infections Infectivity Membrane trafficking Mice Organs Peptides Peptidyl-dipeptidase A Peptidyl-Dipeptidase A - metabolism Protein Binding Protein transport Receptors Receptors, Transferrin - genetics Receptors, Transferrin - metabolism Respiratory diseases SARS-CoV-2 - metabolism Severe acute respiratory syndrome coronavirus 2 Spike Glycoprotein, Coronavirus - metabolism Spike protein Transferrin Transferrins Viral diseases |
title | Human transferrin receptor can mediate SARS-CoV-2 infection |
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