Studies on the Effect of Piperine on Hepatocyte Nuclear Factor 1 Alpha (HNF-1α) and Sterol Regulatory Element-Binding Protein 1c (SREBP-1c) Levels in High-Fat-Diet and Sucrose-Induced Type 2 Diabetes Mellitus Rats

Piperine, a naturally occurring compound in black pepper ( ), is known for its potential health benefits, including its reported enhancement of insulin sensitivity. However, the precise impact of piperine on hepatocyte nuclear factor 1 alpha (HNF-1α) and sterol regulatory element-binding protein 1c...

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Published in:Curēus (Palo Alto, CA) CA), 2024-02, Vol.16 (2), p.e54061-e54061
Main Authors: Zuvairiya, Ummu, Jayaraman, Selvaraj, Sankaran, Kavitha, Veeraraghavan, Vishnu Priya, R, Gayathri
Format: Article
Language:English
Subjects:
Antidiabetics
Bioavailability
Diabetes
Diet
Enzymes
Fatty acids
Genes
Glucose
Homeostasis
Insulin resistance
Internal Medicine
Laboratory animals
Lipids
Liver
Metabolism
Preventive Medicine
Proteins
Sterols
Sucrose
Therapeutics
Transcription factors
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Summary:Piperine, a naturally occurring compound in black pepper ( ), is known for its potential health benefits, including its reported enhancement of insulin sensitivity. However, the precise impact of piperine on hepatocyte nuclear factor 1 alpha (HNF-1α) and sterol regulatory element-binding protein 1c (SREBP-1c), transcription factors for insulin signaling and glucose metabolism in hepatocytes, remains unclear. This study aims to investigate the effect of piperine, compared to metformin, on blood glucose and insulin levels by modifying the expression of hepatic HNF-1α and SREBP-1c in high-fat-diet (HFD) and sucrose-induced type 2 diabetes mellitus (T2DM) rats and in human Chang liver cells. Adult male albino rats were categorized into four groups: group 1 as the control, group 2 as T2DM, group 3 as T2DM rats treated with piperine (40 mg), and group 4 as T2DM rats treated with metformin (50 mg). Fasting blood glucose (FBG) and serum insulin levels were measured using enzyme-linked immunosorbent assay (ELISA), while real-time polymerase chain reaction (RT-PCR) analysis was conducted to assess the mRNA expression of HNF-1α and SREBP-1c. Further, piperine was treated with normal and high glucose-induced Chang liver cells, and gene expression was analysed. Data analysis was performed using one-way analysis of variance (ANOVA), with a significance set at p
ISSN:2168-8184
2168-8184
DOI:10.7759/cureus.54061