Small fibre integrity and axonal pathology in the rat model of experimental autoimmune neuritis

• Published in: Brain communications 2024-03, Vol.6 (2), p.fcae059-fcae059
• Main Authors: Renk, Pia, Sgodzai, Melissa, Klimas, Rafael, Blusch, Alina, Grüter, Thomas, Motte, Jeremias, Pedreiturria, Xiomara, Gebel, Jeannette, Gobrecht, Philipp, Fischer, Dietmar, Gold, Ralf, Pitarokoili, Kalliopi
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 2632-1297; 2632-1297
• DOI: 10.1093/braincomms/fcae059

Abstract Experimental autoimmune neuritis is a common animal model for acute human immune–mediated polyneuropathies. Although already established in 1955, a number of pathophysiological mechanisms remain unknown. In this study, we extensively characterize experimental autoimmune neuritis progression in Lewis rats, including new insights into the integrity of small nerve fibres, neuropathic pain and macrophage activation. Acute experimental autoimmune neuritis was induced with P253–78 peptide and consequently investigated using the gait analysis system CatWalk XT, electrophysiological and histopathological analyses, quantitative polymerase chain reaction (PCR), dorsal root ganglia outgrowth studies, as well as the von Frey hair and Hargreaves tests. For the longitudinal setup, rats were sacrificed at Day (d) 10 (onset), d15 (peak), d26 (recovery) and d29 (late recovery). We confirmed the classical T-cell and macrophage-driven inflammation and the primarily demyelinating nature of the experimental autoimmune neuritis. The dual role of macrophages in experimental autoimmune neuritis is implicated by the high number of remaining macrophages throughout disease progression. Furthermore, different subpopulations of macrophages based on Cx3-motif chemokine receptor 1 (Cx3cr1), platelet factor 4 (Pf4) and macrophage galactose-type lectin-1 (Mgl1) expressions were identified. In addition, modulation of the sensory system in experimental autoimmune neuritis was detected. An outgrowth of small fibres in the plantar skin at the onset and peak of the experimental autoimmune neuritis was evident parallel to the development of acute hyperalgesia mediated through transient receptor potential vanilloid 1 modulation. Our data depict experimental autoimmune neuritis as a primary demyelinating disease with implicated axonal damage, a small unmyelinated fibre impairment throughout the disease progression course, and underline the pivotal role of macrophages in the effector and during the recovery stage. Experimental autoimmune neuritis is a widely used animal model for immune-mediated neuropathies. Renk et al. reported a thorough longitudinal characterization of the disease, revealing novel findings including the altered integrity of small fibres correlating with altered pain sensation and the identification of different macrophage subtypes during the recovery phase correlating with improved regenerative capacity. Graphical Abstract Graphical Abstract