The Bulk Breast Cancer Cell and Breast Cancer Stem Cell Activity of Binuclear Copper(II)‐Phenanthroline Complexes

• Published in: Chemistry : a European journal 2023-08, Vol.29 (45), p.e202301188-n/a
• Main Authors: Osei, Priscilla B., Northcote‐Smith, Joshua, Fang, Jiaxin, Singh, Kuldip, Ortu, Fabrizio, Suntharalingam, Kogularamanan
• Format: Article
• Language: English
• Subjects: Anticancer properties; Antineoplastic Agents - pharmacology; Antitumor agents; antitumour agent; Apoptosis; artificial metallonuclease; bioinorganic chemistry; Breast cancer; Breast Neoplasms - drug therapy; cancer stem cell; Cell Line, Tumor; Chemistry; Chlorides; Cisplatin; Coordination Complexes - pharmacology; Copper; DNA damage; Female; Humans; Ligands; Neoplastic Stem Cells; Phenanthrolines - pharmacology; Reactive oxygen species; Salinomycin; Stem cells; Toxicity
• ISSN: 0947-6539; 1521-3765
• DOI: 10.1002/chem.202301188

Mononuclear copper(II)‐phenanthroline complexes have been widely investigated as anticancer agents whereas multinuclear copper(II)‐phenanthroline complexes are underexplored. Here the synthesis and characterisation of two new binuclear copper(II)‐phenanthroline complexes 1 and 2 is reported, comprising of 2,9‐dimethyl‐1,10‐phenanthroline or 2,9‐dimethyl‐4,7‐diphenyl‐1,10‐phenanthroline, terminal chloride ligands, and bridging chloride or hydroxide ligands. The binuclear copper(II) complex containing 2,9‐dimethyl‐1,10‐phenanthroline 1 displays nanomolar toxicity towards bulk breast cancer cells and breast cancer stem cells (CSCs) grown in monolayers, >50‐fold greater than cisplatin (an anticancer metallodrug) and salinomycin (a gold‐standard anti‐CSC agent). Spectacularly, 1 exhibits >100‐fold greater potency toward three‐dimensionally cultured mammospheres than cisplatin and salinomycin. Mechanistic studies show that 1 evokes breast CSC apoptosis by elevating intracellular reactive oxygen species levels and damaging genomic DNA (possibly by an oxidative mechanism). To the best of our knowledge, this is the first study to probe the anti‐breast CSC properties of binuclear copper(II)‐phenanthroline complexes. Investigations of the anti‐cancer stem cell (CSC) properties of multinuclear metal complexes are rare. Here the synthesis, characterisation, and bulk breast cancer cell and breast CSC activity of two new binuclear copper(II) complexes are reported. The mechanism of action of the most stable and potent binuclear copper(II) complex were elucidated.