Platinum(II)-Based Optical Probes for Imaging Quadruplex DNA Structures via Phosphorescence Lifetime Imaging Microscopy

G-quadruplex DNA is a non-canonical structure that forms in guanine-rich regions of the genome. There is increasing evidence showing that G-quadruplexes have important biological functions, and therefore molecular tools to visualise these structures are important. Herein we report on a series of new...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2023-10, Vol.62 (42), p.e202310402-e202310402
Main Authors: Berrones Reyes, Jessica, Sherin, Peter S, Sarkar, Amrita, Kuimova, Marina K, Vilar, Ramon
Format: Article
Language:English
Subjects:
Binding
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA probes
G-Quadruplexes
Genomes
Imaging
Microscopy
Phosphorescence
Platinum
Platinum - chemistry
Probes
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Summary:G-quadruplex DNA is a non-canonical structure that forms in guanine-rich regions of the genome. There is increasing evidence showing that G-quadruplexes have important biological functions, and therefore molecular tools to visualise these structures are important. Herein we report on a series of new cyclometallated platinum(II) complexes which, upon binding to G-quadruplex DNA, display an increase in their phosphorescence, acting as switch-on probes. More importantly, upon binding to G-quadruplexes they display a selective and distinct lengthening of their emission lifetime. We show that this effect can be used to selectively visualise these structures in cells using Phosphorescence Lifetime Imaging Microscopy (PLIM).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202310402