Assessment of various standard fish diets on gut microbiome of platyfish Xiphophorus maculatus

Diet is an external factor that affects the physiological baseline of research animals. It can shape gut microbiome, which can impact the host. As a result, dietary variation can challenge experimental reproducibility and data integration across studies when not appropriately considered. To control...

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Bibliographic Details
Published in:Journal of experimental zoology. Part B, Molecular and developmental evolution Molecular and developmental evolution, 2024-05, Vol.342 (3), p.271-277
Main Authors: Soria, Erika, Russo, Crystal, Carlos‐Shanley, Camila, Drewery, Merritt, Boswell, Will, Savage, Markita, Sanchez, Lindsey, Chang, Carolyn, Varga, Zoltan M., Kent, Michael L., Sharpton, Thomas J., Lu, Yuan
Format: Article
Language:English
Subjects:
Animal Feed
Animals
Cyprinodontiformes
Diet - veterinary
Gastrointestinal Microbiome - physiology
gut microbiome
husbandry
RNA, Ribosomal, 16S - genetics
standardized diet
Xiphophorus
Zebrafish - microbiology
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Summary:Diet is an external factor that affects the physiological baseline of research animals. It can shape gut microbiome, which can impact the host. As a result, dietary variation can challenge experimental reproducibility and data integration across studies when not appropriately considered. To control for diet‐induced variation, reference diets have been developed for common biomedical models. However, such reference diets have not yet been developed for nontraditional model organisms, such as Xiphophorus species. In this study, we compared two diets designed for zebrafish, a commercial zebrafish diet (Gemma and GEM), and a proposed zebrafish reference diet developed by the Watts laboratory at the University of Alabama at Birmingham (WAT) to the Xiphophorus Genetic Stock Center custom diet (CON) to evaluate the influence of diet on the Xiphophorus gut microbiome. Xiphophorus maculatus were fed the three diets from 2 to 6 months of age. Feces were collected and the gut microbiome was assessed using 16S rRNA sequencing every month. We observed substantial diet‐driven variation in the gut microbiome. Our results indicate that diets developed specifically for zebrafish can affect the gut microbiome composition and may not be optimal for Xiphophorus. Diet can lead to substantial variation in the fish gut microbiome. Research Highlights Zebrafish diets change Xiphophorus gut microbiome; Species specific standardized diets need to be developed.
ISSN:1552-5007
1552-5015
DOI:10.1002/jez.b.23218