Pink1 deficiency enhances neurological deficits and inflammatory responses after intracerebral hemorrhage in mice

• Published in: Neurotherapeutics 2024-03, Vol.21 (2), p.e00317-e00317, Article e00317
• Main Authors: Li, Jingchen, Li, Jianliang, Guo, Erkun, Wang, Yuanyu, Yang, Ming, Huo, Haoran, Shi, Yunpeng, Zhao, Lin
• Format: Article
• Language: English
• Subjects: Animals; Brain - metabolism; Cerebral Hemorrhage - complications; Cerebral Hemorrhage - genetics; Cerebral Hemorrhage - metabolism; Inflammation; Interleukin-10; Intracerebral hemorrhage; Mice; Mice, Inbred C57BL; Neurological deficits; Original; Pink1; Tumor Necrosis Factor-alpha - metabolism; Water - metabolism
• ISSN: 1878-7479; 1933-7213; 1878-7479
• DOI: 10.1016/j.neurot.2024.e00317

Pink1 (PTEN-induced putative kinase 1) is a protein associated with maintaining mitochondrial function and integrity and has been reported to mediate neurodegeneration and neuroinflammation. While the role of Pink1 in intracerebral hemorrhage (ICH)-related neurological deficits and inflammatory responses is not deciphered. Congenic blood was transfused into the left corpus striatum to construct the ICH model in C57/BL6 wild-type (WT) and Pink1−/− mice. The relative expression of Pink1, monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-2, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, Cd86, nitric oxide synthase 2 (Nos2), Cd206, arginase 1 (Arg-1), and IL-10 was detected with qRT-PCR, Western blotting, or ELISA. Mouse neurological deficit scores (mNSS) and water content were detected, and an open-field test was performed to assay anxiety-like behavior. Remarkably decreased Pink1 expression and increased MIP-2, IL-1β, MCP-1, and TNF-α expression were observed after 12 ​h, 24 ​h, 48 ​h, 72 ​h, and 7 ​d post-ICH induction in the ipsilateral injury hemispheres. Pink1 deficiency could further up-regulate mNSS scores, brain water content, MIP-2, MCP-1, IL-1β, and TNF-α in the ipsilateral injury hemispheres. On the other hand, Pink1 deficiency could decrease the number of center cross, the velocity, and the total distance traveled in open field test. Pink1 deficiency could further up-regulate the mRNA levels of pro-inflammatory (M1) molecules (Cd86, Nos2), and down-regulate the relative expression of anti-inflammatory (M2) molecules (Cd206, Arg-1, and IL-10). Pink1 deficiency deteriorates neurological deficits and inflammatory responses after ICH, which can be considered as a treatment target.