Characterizing a standardized BioPart for BAG-specific expression in C. elegans

Biological parts (BioParts) are modular and standardized DNA sequences that encode biological functions and contribute to the efficient biological engineering of complex systems. Here, we characterize a short BioPart (P , 300 bp) for bright multicopy and single-copy BAG-specific expression starting...

Full description

Saved in:
Bibliographic Details
Published in:microPublication biology 2024, Vol.2024
Main Authors: Qian, Xinglin, AlHarbi, Sarah, Frøkjær-Jensen, Christian
Format: Article
Language:English
Subjects:
C. Elegans
Expression Data
New Finding
Software
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Biological parts (BioParts) are modular and standardized DNA sequences that encode biological functions and contribute to the efficient biological engineering of complex systems. Here, we characterize a short BioPart (P , 300 bp) for bright multicopy and single-copy BAG-specific expression starting from the gastrula stage in hermaphrodite and male . We have generated standardized P cloning vectors for BAG-specific and expression compatible with extra-chromosomal arrays and for single-copy transgene insertion. The short P promoter is easy to generate by gene synthesis and has been incorporated into our online transgene design tool (www.wormbuilder.org/transgenebuilder ) .
ISSN:2578-9430
DOI:10.17912/micropub.biology.001150