Plasma microRNA-320c as a Potential Biomarker for the Severity of Knee Osteoarthritis and Regulates cAMP Responsive Element Binding Protein 5 (CREB5) in Chondrocytes

Objective. Osteoarthritis (OA) is a commonly known prevalent joint disease, with limited therapeutic methods. This study aimed to investigate the expression of plasma microRNA-320c (miR-320c) in patients with knee OA and to explore the clinical value and potential mechanism of miR-320c in knee OA. M...

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Bibliographic Details
Published in:Disease markers 2024, Vol.2024, p.9936295-10
Main Authors: Zhou, Rongwei, Zhao, Like, Wang, Qian, Cheng, Yongjing, Song, Miao, Huang, Cibo
Format: Article
Language:English
Subjects:
3' Untranslated regions
Arthritis
Binding sites
Biomarkers
Biomarkers - metabolism
Biotechnology
c-Fos protein
c-Jun protein
Cartilage
Chondrocytes
Chondrocytes - metabolism
Correlation analysis
Cyclic AMP
Cyclic AMP Response Element-Binding Protein A - metabolism
Cytokines
Diagnostic systems
Disease
Enzyme-linked immunosorbent assay
Experiments
Health care
Humans
Inflammation
Informed consent
Interleukin-1beta - metabolism
Joint diseases
Knee
Luciferases - metabolism
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Osteoarthritis
Osteoarthritis, Knee - genetics
Osteoarthritis, Knee - metabolism
Pathogenesis
Plasma
Plasmids
Proteins
Ribonucleic acid
RNA
Statistical analysis
Transcription factors
Tumor necrosis factor-TNF
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Summary:Objective. Osteoarthritis (OA) is a commonly known prevalent joint disease, with limited therapeutic methods. This study aimed to investigate the expression of plasma microRNA-320c (miR-320c) in patients with knee OA and to explore the clinical value and potential mechanism of miR-320c in knee OA. Methods. Forty knee OA patients and 20 healthy controls were enrolled. The levels of plasma miR-320c and plasma inflammatory cytokines were measured by real-time PCR or ELISA. Correlations of Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores and cytokine levels with the miR-320c expression level were evaluated by Pearson correlation analysis. Then, a receiver operating characteristic (ROC) curve was drawn to analyse the diagnostic value of miR-320c in OA. Finally, the interaction of miR-320c and cAMP responsive element binding protein 5 (CREB5) was determined using a luciferase reporter assay, and the effect of CREB5 on the cAMP pathway was assessed. Results. The expression level of plasma miR-320c was significantly higher in OA patients than in healthy controls (p 
ISSN:0278-0240
1875-8630
DOI:10.1155/2024/9936295