A Cross-Sectional Quantitative Metabolomics Study Evidencing the Metabolic Signature in Six Organs during a 14-Week High-Fat High-Sucrose and Standard Diet in Mice

• Published in: Nutrients 2024-03, Vol.16 (6), p.803
• Main Authors: Drevet Mulard, Eva, Guibert, Sylvie, Mey, Anne, Lefevre, Camille, Chauvin, Marie-Agnès, Pinteur, Claudie, Monet, Marie-Ambre, Godet, Murielle, Madec, Anne-Marie, Morio, Béatrice, Rieusset, Jennifer, Rautureau, Gilles J P, Panthu, Baptiste
• Format: Article
• Language: English
• Subjects: Amino acids; Animals; Body fat; Brain; Cross-Sectional Studies; Diabetes; Diabetes Mellitus, Type 2 - complications; Diet; Diet, High-Fat - adverse effects; Discriminant analysis; Experiments; Fourier transforms; Glucose; Insulin; Kidneys; Laboratory animals; Life Sciences; Liver; Liver - metabolism; Male; Metabolism; Metabolites; Metabolomics; Mice; Mice, Inbred C57BL; Muscles; Musculoskeletal system; NMR; Nuclear magnetic resonance; Obesity; Obesity - metabolism; Overweight; Physiological aspects; Risk factors; Sexually transmitted diseases; Software; Spectrum analysis; Sucrose; Sucrose - metabolism; Type 2 diabetes; Variance analysis
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16060803

Obesity is a risk factor for many diseases, such as type 2 diabetes and cardiovascular diseases. In line with the need for precision medicine, the search for biomarkers reporting the progression of obesity- and diet-associated disorders is urgent. We used NMR to determine the metabolomics profile of key organs (lung, liver, heart, skeletal muscle, kidney, and brain) and serum from male C57Bl/6J mice (5 weeks old) fed for 6, 10, and 14 weeks on a high-fat and high-sucrose diet (HFHSD) vs. a standard diet (STD). We determined metabolite concentrations in the organs at each time point, which allowed us to discriminate age- and diet-related effects as well as the interactions between both, highlighting the need to evaluate the influence of age as a confounding factor on metabolic signatures. Notably, the analysis revealed the influence of time on metabolite concentrations in the STD condition, probably reflecting the juvenile-to-adult transition. Variations impacted the liver and lung metabolites, revealing the strong influence of the HFHS diet on normal metabolism maturation during youth.