Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water

Conventional methods for pathogen detection in water rely on time-consuming enrichment steps followed by biochemical identification strategies, which require assay times ranging from 24 hours to a week. However, in recent years, significant efforts have been made to develop biosensing technologies e...

Full description

Saved in:
Bibliographic Details
Published in:Polymers 2024-03, Vol.16 (6), p.861
Main Authors: Lopes, Rafaela Nascimento, Pinto, Paulo Henrique Silva, Vargas, Juan David Lopez, Dante, Alex, Macrae, Andrew, Allil, Regina Célia Barros, Werneck, Marcelo Martins
Format: Article
Language:English
Subjects:
Analysis
Antibodies
Antigens
Bacteria
Biosensors
Configurations
E coli
Enzymes
Escherichia coli
Fiber optics
Immunosensors
Methods
Optical fibers
Optics
Optoelectronics
Pathogens
Sensitivity
Sensors
Severe acute respiratory syndrome coronavirus 2
Thin films
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Conventional methods for pathogen detection in water rely on time-consuming enrichment steps followed by biochemical identification strategies, which require assay times ranging from 24 hours to a week. However, in recent years, significant efforts have been made to develop biosensing technologies enabling rapid and close-to-real-time detection of waterborne pathogens. In previous studies, we developed a plastic optical fiber (POF) immunosensor using an optoelectronic configuration consisting of a U-Shape probe connected to an LED and a photodetector. Bacterial detection was evaluated with the immunosensor immersed in a bacterial suspension in water with a known concentration. Here, we report on the sensitivity of a new optoelectronic configuration consisting of two POF U-shaped probes, one as the reference and the other as the immunosensor, for the detection of . In addition, another methos of detection was tested where the sensors were calibrated in the air, before being immersed in a bacterial suspension and then read in the air. This modification improved sensor sensitivity and resulted in a faster detection time. After the immunocapture, the sensors were DAPI-stained and submitted to confocal microscopy. The histograms obtained confirmed that the responses of the immunosensors were due to the bacteria. This new sensor detected the presence of at 10 CFU/mL in less than 20 min. Currently, sub-20 min is faster than previous studies using fiber-optic based biosensors. We report on an inexpensive and faster detection technology when compared with conventional methods.
ISSN:2073-4360
2073-4360
DOI:10.3390/polym16060861