Functional Inhibition of Katanin Affects Synaptic Plasticity

Dynamic microtubules critically regulate synaptic functions, but the role of microtubule severing in these processes is barely understood. Katanin is a neuronally expressed microtubule-severing complex regulating microtubule number and length in cell division or neurogenesis; however, its potential...

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Bibliographic Details
Published in:The Journal of neuroscience 2024-03, Vol.44 (13), p.e0374232023
Main Authors: Lombino, Franco L, Schwarz, Jürgen R, Pechmann, Yvonne, Schweizer, Michaela, Jark, Rebecca, Stange, Oliver, Glatzel, Markus, Gee, Christine E, Hausrat, Torben J, Gromova, Kira V, Kneussel, Matthias
Format: Article
Language:English
Subjects:
Animals
Cell division
Dendrites
Dendritic spines
Functional plasticity
Katanin - genetics
Katanin - metabolism
Long-term potentiation
Mice
Microtubules
Microtubules - metabolism
Neurogenesis
Neuronal Plasticity
Neurons - physiology
Structure-function relationships
Synapses
Synaptic plasticity
α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid
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Summary:Dynamic microtubules critically regulate synaptic functions, but the role of microtubule severing in these processes is barely understood. Katanin is a neuronally expressed microtubule-severing complex regulating microtubule number and length in cell division or neurogenesis; however, its potential role in synaptic functions has remained unknown. Studying mice from both sexes, we found that katanin is abundant in neuronal dendrites and can be detected at individual excitatory spine synapses. Overexpression of a dominant-negative ATPase-deficient katanin subunit to functionally inhibit severing alters the growth of microtubules in dendrites, specifically at premature but not mature neuronal stages without affecting spine density. Notably, interference with katanin function prevented structural spine remodeling following single synapse glutamate uncaging and significantly affected the potentiation of AMPA-receptor-mediated excitatory currents after chemical induction of long-term potentiation. Furthermore, katanin inhibition reduced the invasion of microtubules into fully developed spines. Our data demonstrate that katanin-mediated microtubule severing regulates structural and functional plasticity at synaptic sites.
ISSN:0270-6474
1529-2401
DOI:10.1523/JNEUROSCI.0374-23.2023