Impairments of retinal hemodynamics and oxygen metrics in ocular hypertension-induced ischemia-reperfusion

Ischemia-reperfusion (I/R) is an established model for retinal neurodegeneration. However, there is limited knowledge of retinal physiological metrics and their relationships to retinal function and morphology in the I/R model. The purpose of the study was to test the hypotheses that retinal hemodyn...

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Bibliographic Details
Published in:Experimental eye research 2022-12, Vol.225, p.109278-109278, Article 109278
Main Authors: Rahimi, Mansour, Leahy, Sophie, Matei, Nathanael, Burford, James, Blair, Norman P., Shahidi, Mahnaz
Format: Article
Language:English
Subjects:
Animals
Benchmarking
Blood flow
Disease Models, Animal
Electroretinography
Glaucoma
Hemodynamics
Ischemia - metabolism
Ischemia-reperfusion
Ocular Hypertension - metabolism
Oxygen - metabolism
Oxygen delivery
Rats
Reperfusion
Reperfusion Injury - metabolism
Retina - metabolism
Retinal thickness
Visual function
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Summary:Ischemia-reperfusion (I/R) is an established model for retinal neurodegeneration. However, there is limited knowledge of retinal physiological metrics and their relationships to retinal function and morphology in the I/R model. The purpose of the study was to test the hypotheses that retinal hemodynamic and oxygen metrics are impaired and associated with visual dysfunction, retinal thinning, and retinal ganglion cell (RGC) loss due to I/R injury. Intraocular pressure (IOP) was increased in one eye of 10 rats for 90 min followed by reperfusion. Fellow eyes served as controls. After one week of reperfusion, multimodal imaging was performed to quantify total retinal blood flow (TRBF) and retinal vascular oxygen contents. Retinal oxygen delivery (DO2) and metabolism (MO2) were calculated. Pattern-evoked electroretinography (PERG) and optical coherence tomography were performed to measure RGC function and retinal thicknesses, respectively. RGCs were counted from retina whole mounts. After one week of reperfusion, TRBF was lower in study eyes than in control eyes (p 
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2022.109278